Advances in adaptive optics–based two-photon fluorescence microscopy for brain imaging

P Sahu, N Mazumder - Lasers in medical science, 2020 - Springer
Deep tissue imaging using two-photon fluorescence (TPF) techniques have revolutionized
the optical imaging community by providing in depth molecular information at the single-cell …

[PDF][PDF] Two-photon fluorescence microscopy: basic principles, advantages and risks

SJ Mulligan, BA MacVicar, A Méndez-Vilas… - Modern Research and …, 2007 - Citeseer
The application of two-photon excitation to fluorescence microscopy has become a powerful
tool for studying biological function in live tissue and offers many advantages over …

In vivo three-photon microscopy of subcortical structures within an intact mouse brain

NG Horton, K Wang, D Kobat, CG Clark, FW Wise… - Nature …, 2013 - nature.com
Two-photon fluorescence microscopy enables scientists in various fields including
neuroscience,, embryology and oncology to visualize in vivo and ex vivo tissue morphology …

[HTML][HTML] Characterization of red fluorescent reporters for dual-color in vivo three-photon microscopy

MA Thornton, GL Futia, ME Stockton… - …, 2022 - spiedigitallibrary.org
Significance: Three-photon (3P) microscopy significantly increases the depth and resolution
of in vivo imaging due to decreased scattering and nonlinear optical sectioning …

[HTML][HTML] Three-photon excited fluorescence imaging in neuroscience: From principles to applications

Y Xiao, P Deng, Y Zhao, S Yang, B Li - Frontiers in Neuroscience, 2023 - frontiersin.org
The development of three-photon microscopy (3PM) has greatly expanded the capability of
imaging deep within biological tissues, enabling neuroscientists to visualize the structure …

Exploiting the potential of commercial objectives to extend the field of view of two-photon microscopy by adaptive optics

J Yao, Y Gao, Y Yin, P Lai, S Ye, W Zheng - Optics letters, 2022 - opg.optica.org
Two-photon microscopy (TPM) has provided critical in situ and in vivo information in
biomedical studies due to its high resolution, intrinsic optical sectioning, and deep …

Recent advances in two-photon imaging: technology developments and biomedical applications

Y Chen, H Guo, W Gong, L Qin, H Aleyasin… - Chinese Optics …, 2013 - opg.optica.org
In the past two decades, two-photon microscopy (TPM) transforms biomedical research,
allowing nondestructive high-resolution fluorescent molecular imaging and label-free …

Extending the fundamental imaging-depth limit of multi-photon microscopy by imaging with photo-activatable fluorophores

Z Chen, L Wei, X Zhu, W Min - Optics express, 2012 - opg.optica.org
It is highly desirable to be able to optically probe biological activities deep inside live
organisms. By employing a spatially confined excitation via a nonlinear transition …

[HTML][HTML] Three-photon adaptive optics for mouse brain imaging

D Sinefeld, F Xia, M Wang, T Wang, C Wu… - Frontiers in …, 2022 - frontiersin.org
Three-photon microscopy (3PM) was shown to allow deeper imaging than two-photon
microscopy (2PM) in scattering biological tissues, such as the mouse brain, since the longer …

[HTML][HTML] Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo

Z Qin, S He, C Yang, JSY Yung, C Chen… - Light: Science & …, 2020 - nature.com
In vivo fundus imaging offers non-invasive access to neuron structures and biochemical
processes in the retina. However, optical aberrations of the eye degrade the imaging …