The design of enzyme catalytic stability is of great significance in medicine and industry.
However, traditional methods are time-consuming and costly. Hence, a growing number of …

Thermal stability is one of the most important properties of enzymes, which sustains life and
determines the potential for the industrial application of biocatalysts. Although traditional …

Natural enzymes are delicate biomolecules possessing only marginal thermodynamic
stability. Poorly stable, misfolded, and aggregated proteins lead to huge economic losses in …

The combinatorial space of an enzyme sequence has astronomical possibilities and
exploring it with contemporary experimental techniques is arduous and often ineffective …

The fundamentals of thermostability engineering need to be carried out for proteins with low
thermal stability to expand their utilization. Thus, comprehension of the thermal stability …

The potential of engineered enzymes in industrial applications is often limited by their
expression levels, thermal stability, and catalytic diversity. De novo enzyme design faces …

Although computational enzyme design is of great importance, the advances utilizing
physics-based approaches have been slow, and further progress is urgently needed. One …

Predicting the thermostability of a biomolecule, given its sequence, is one of the big
challenges of protein engineering and developing tools to screen thermostable mutants is of …

The ability of proteins and enzymes to maintain a functionally active conformation under
adverse environmental conditions is an important feature of biocatalysts, vaccines, and …

The local flexibility of an enzyme's active center plays pivotal roles in catalysis, however,
little is known about how the flexibility of these flexible residues affects stability. In this study …