Additivities for Soluble Recombinant Protein Expression in Cytoplasm of Escherichia coli

DL Atroshenko, EP Sergeev, DI Golovina, AA Pometun - Fermentation, 2024 - mdpi.com
Recombinant protein expression in Escherichia coli is a fundamental technique in molecular
biology and biotechnology. This review provides a comprehensive overview of various …

Strategies for optimization of heterologous protein expression in E. coli: Roadblocks and reinforcements

J Kaur, A Kumar, J Kaur - International journal of biological …, 2018 - Elsevier
E. coli is most preferred system used for the production of recombinant proteins in bacteria
and the availability of improved genetic tools/methods are making it more valuable than …

Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli

HP Sørensen, KK Mortensen - Microbial cell factories, 2005 - Springer
Pure, soluble and functional proteins are of high demand in modern biotechnology. Natural
protein sources rarely meet the requirements for quantity, ease of isolation or price and …

Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli

A Eskandari, NG Nezhad, TC Leow… - Archives of …, 2024 - Springer
Producing recombinant proteins is a major accomplishment of biotechnology in the past
century. Heterologous hosts, either eukaryotic or prokaryotic, are used for the production of …

[PDF][PDF] An overview of the parameters for recombinant protein expression in Escherichia coli

BC Joseph, S Pichaimuthu, S Srimeenakshi… - J Cell Sci …, 2015 - researchgate.net
Escherichia coli, is one of the most widely preferred organism for the production of
recombinant protein. Most of the FDA approved therapeutic proteins are produced in E. coli …

Extracellular recombinant protein production from Escherichia coli

Y Ni, R Chen - Biotechnology letters, 2009 - Springer
Escherichia coli is the most commonly used host for recombinant protein production and
metabolic engineering. Extracellular production of enzymes and proteins is advantageous …

Tunable recombinant protein expression with E. coli in a mixed-feed environment

P Sagmeister, C Schimek, A Meitz, C Herwig… - Applied microbiology …, 2014 - Springer
Controlling the recombinant protein production rate in Escherichia coli is of utmost
importance to ensure product quality and quantity. Up to now, only the genetic construct …

An overview of culture conditions for recombinant protein expression in Escherichia coli

IP Maksum, FPU Latifah, A Nabiel… - Journal of Applied …, 2023 - biotechrep.ir
Escherichia coli is one of the most commonly used organisms for producing recombinant
protein. The recognized cell genome and well-known cell factory of the bacteria make it an …

Recombinant protein expression in Escherichia coli: advances and challenges

GL Rosano, EA Ceccarelli - Frontiers in microbiology, 2014 - frontiersin.org
Escherichia coli is one of the organisms of choice for the production of recombinant proteins.
Its use as a cell factory is well-established and it has become the most popular expression …

[PDF][PDF] Advances and challenges in recombinant protein expression in Escherichia coli

P Saeedi, E Behzadi, R Kazemi, MJ Rezaei… - New Cellular and …, 2019 - ncmbjpiau.ir
Escherichia coli is the first bacteria on which cloning and protein expression are carried out.
The purpose of this study was to summarize the numerous articles published in the field of …