Methods of sperm preparation for assisted reproduction.

D Mortimer, ST Mortimer - Annals of the Academy of Medicine …, 1992 - europepmc.org
Because seminal plasma contains factors which inhibit the fertilising ability of spermatozoa,
it is essential that spermatozoa be separated from it quickly and efficiently. Four basic …

Sperm preparation for ART

RR Henkel, WB Schill - Reproductive biology and endocrinology, 2003 - Springer
The onset of clinical assisted reproduction, a quarter of a century ago, required the isolation
of motile spermatozoa. As the indication of assisted reproduction shifted from mere …

[HTML][HTML] Sperm preparation: state-of-the-art—physiological aspects and application of advanced sperm preparation methods

R Henkel - Asian journal of andrology, 2012 - ncbi.nlm.nih.gov
For assisted reproduction technologies (ART), numerous techniques were developed to
isolate spermatozoa capable of fertilizing oocytes. While early methodologies only focused …

Separation of motile human spermatozoa by means of a glass bead column

S Daya, RBL Gwatkin, H Bissessar - Gamete research, 1987 - Wiley Online Library
Fertility potential of semen depends upon the presence of viable and motile spermatozoa. A
variety of techniques has been reported whereby motile spermatozoa are separated from …

A 'migration-gravity sedimentation'method for collecting motile spermatozoa from human semen

NT Tea, M Jondet, R Scholler - In Vitro Fertilizȧtion, Embryo Transfer and …, 1984 - Springer
Methods of separating motile human spermatozoa avoiding centrifugation have been
described 1–3. Ejaculated semen is deposited in contact with the culture medium, and then …

Sperm preparation techniques

H Bourne, J Archer - Textbook of assisted reproductive techniques, 2017 - taylorfrancis.com
OVERVIEW e aim of sperm preparation for assisted reproduction technology (ART) is to
maximize the chances of fertilization to provide as many normally fertilized oocytes as …

Improvement of sperm quality in abnormal semen samples using a modified swim-up procedure

P Andolz, MA Bielsa, A Genesca, J Benet… - Human …, 1987 - academic.oup.com
A modified swim-up procedure for the selection of motile and morphologically normal
spermatozoa is described. Applied to abnormal (astheno and asthenoterato) semen …

Comparison of centrifugation-and noncentrifugation-based techniques for recovery of motile human sperm in assisted reproduction

ES Sills, KM Wittkowski, MJ Tucker, M Perloe… - Archives of …, 2002 - Taylor & Francis
To compare standard density gradient/centrifugation sperm preparation with a novel non-
centrifugation-based dual-chamber capillary dish in efficiency for motile human sperm …

Comparison of four methods for sperm preparation for IUI

SS Ren, GH Sun, CH Ku, DC Chen, GJ Wu - Archives of andrology, 2004 - Taylor & Francis
We compared the pregnancy rates and sperm characteristics of semen prepared by the
albumin, Percoll, Puresperm, and swim-up methods. Semen analysis was performed by a …

Improved techniques for separating motile spermatozoa from human semen: II. An atraumatic centrifugation method

A Makler, O Murillo, G Huszar… - … journal of andrology, 1984 - Wiley Online Library
A simple and atraumatic method for concentrating washed, motile spermatozoa from normal
and subnormal semen specimens is described. It incorporates a modified technique of …