Regulation of leaderless mRNA translation in bacteria

LE Leiva, A Katz - Microorganisms, 2022 - mdpi.com
In bacteria, the translation of genetic information can begin through at least three different
mechanisms: canonical or Shine-Dalgarno-led initiation, readthrough or 70S scanning …

An AUG initiation codon, not codon–anticodon complementarity, is required for the translation of unleadered mRNA in Escherichia coli

WJ Van Etten, GR Janssen - Molecular microbiology, 1998 - Wiley Online Library
We determined the in vivo translational efficiency of 'unleadered'lacZ compared with a
conventionally leadered lacZ with and without a Shine–Dalgarno (SD) sequence in …

Leaderless mRNAs in bacteria: surprises in ribosomal recruitment and translational control

I Moll, S Grill, CO Gualerzi, U Bläsi - Molecular microbiology, 2002 - Wiley Online Library
It is commonly believed that the translational efficiency of prokaryotic mRNAs is intrinsically
determined by both primary and secondary structures of their translational initiation regions …

Translational initiation in E. coli occurs at the correct sites genome-wide in the absence of mRNA-rRNA base-pairing

K Saito, R Green, AR Buskirk - Elife, 2020 - elifesciences.org
Shine-Dalgarno (SD) motifs are thought to play an important role in translational initiation in
bacteria. Paradoxically, ribosome profiling studies in E. coli show no correlation between the …

Leaderless mRNAs in the spotlight: ancient but not outdated!

HJ Beck, I Moll - Regulating with RNA in Bacteria and Archaea, 2018 - Wiley Online Library
In bacteria and archaea, translation initiates with a 30S ribosomal subunit interacting with an
initiator tRNA at the ribosome binding site on a canonical mRNA to form a stable translation …

Translation initiation with 70S ribosomes: an alternative pathway for leaderless mRNAs

I Moll, G Hirokawa, MC Kiel, A Kaji… - Nucleic acids …, 2004 - academic.oup.com
It is generally accepted that translation in bacteria is initiated by 30S ribosomal subunits. In
contrast, several lines of rather indirect in vitro evidence suggest that 70S monosomes are …

Evidence against an interaction between the mRNA downstream box and 16S rRNA in translation initiation

I Moll, M Huber, S Grill, P Sairafi, F Mueller… - Journal of …, 2001 - Am Soc Microbiol
Based on the complementarity of the initial coding region (downstream box [db]) of several
bacterial and phage mRNAs to bases 1469 to 1483 in helix 44 of 16S rRNA (anti …

Translation initiation factor 3 antagonizes authentic start codon selection on leaderless mRNAs

K Tedin, I Moll, S Grill, A Resch… - Molecular …, 1999 - Wiley Online Library
In this study, we have examined the influence of initiation factors on translation initiation of
leaderless mRNAs whose 5′‐terminal residues are the A of the AUG initiating codon. A 1: 1 …

Evidence for the translation initiation of leaderless mRNAs by the intact 70 S ribosome without its dissociation into subunits in eubacteria

T Udagawa, Y Shimizu, T Ueda - Journal of Biological Chemistry, 2004 - ASBMB
In eubacteria, the dissociation of the 70 S ribosome into the 30 S and 50 S subunits is the
essential first step for the translation initiation of canonical mRNAs that possess 5′-leader …

Local absence of secondary structure permits translation of mRNAs that lack ribosome-binding sites

LB Scharff, L Childs, D Walther, R Bock - PLoS genetics, 2011 - journals.plos.org
The initiation of translation is a fundamental and highly regulated process in gene
expression. Translation initiation in prokaryotic systems usually requires interaction between …