Enzyme promiscuity: a mechanistic and evolutionary perspective
OKDS Tawfik - Annual review of biochemistry, 2010 - annualreviews.org
Many, if not most, enzymes can promiscuously catalyze reactions, or act on substrates, other
than those for which they evolved. Here, we discuss the structural, mechanistic, and …
than those for which they evolved. Here, we discuss the structural, mechanistic, and …
Enzyme promiscuity: evolutionary and mechanistic aspects
O Khersonsky, C Roodveldt, DS Tawfik - Current opinion in chemical …, 2006 - Elsevier
The past few years have seen significant advances in research related to the 'latent skills' of
enzymes—namely, their capacity to promiscuously catalyze reactions other than the ones …
enzymes—namely, their capacity to promiscuously catalyze reactions other than the ones …
An evolutionary biochemist's perspective on promiscuity
SD Copley - Trends in biochemical sciences, 2015 - cell.com
Evolutionary biochemists define enzyme promiscuity as the ability to catalyze secondary
reactions that are physiologically irrelevant, either because they are too inefficient to affect …
reactions that are physiologically irrelevant, either because they are too inefficient to affect …
Enzyme promiscuity: mechanism and applications
K Hult, P Berglund - Trends in biotechnology, 2007 - cell.com
Introductory courses in biochemistry teach that enzymes are specific for their substrates and
the reactions they catalyze. Enzymes diverging from this statement are sometimes called …
the reactions they catalyze. Enzymes diverging from this statement are sometimes called …
Catalytic promiscuity and the evolution of new enzymatic activities
PJ O'Brien, D Herschlag - Chemistry & biology, 1999 - cell.com
Several contemporary enzymes catalyze alternative reactions distinct from their normal
biological reactions. In some cases the alternative reaction is similar to a reaction that is …
biological reactions. In some cases the alternative reaction is similar to a reaction that is …
Shining a light on enzyme promiscuity
SD Copley - Current opinion in structural biology, 2017 - Elsevier
Highlights•Screening efforts have revealed enzymes with over 100 promiscuous
activities.•High-throughput screening for promiscuous activities can be accomplished in …
activities.•High-throughput screening for promiscuous activities can be accomplished in …
[HTML][HTML] Enzyme evolution: innovation is easy, optimization is complicated
Highlights•Enzyme 'promiscuity space'is vast.•Functional innovation by divergent evolution
is easy.•Gene loss is also an important driver of enzyme evolution.•Evolutionary trajectories …
is easy.•Gene loss is also an important driver of enzyme evolution.•Evolutionary trajectories …
A bird's-eye view of enzyme evolution: chemical, physicochemical, and physiological considerations
Enzymes catalyze a vast range of reactions. Their catalytic performances, mechanisms,
global folds, and active-site architectures are also highly diverse, suggesting that enzymes …
global folds, and active-site architectures are also highly diverse, suggesting that enzymes …
Protein promiscuity and its implications for biotechnology
I Nobeli, AD Favia, JM Thornton - Nature biotechnology, 2009 - nature.com
Molecular recognition between proteins and their interacting partners underlies the
biochemistry of living organisms. Specificity in this recognition is thought to be essential …
biochemistry of living organisms. Specificity in this recognition is thought to be essential …
[HTML][HTML] Cooperativity and flexibility in enzyme evolution
A Pabis, VA Risso, JM Sanchez-Ruiz… - Current opinion in …, 2018 - Elsevier
Highlights•Cooperativity and flexibility play important roles in enzyme evolution.•
Evolutionarily-important flexibility can be both structural or electrostatic.•Floppiness in …
Evolutionarily-important flexibility can be both structural or electrostatic.•Floppiness in …