Understanding complex biological systems requires visualizing structures and processes
deep within living organisms. We developed a compact adaptive optics module and …

Multiphoton microscopy has become a powerful tool with which to visualize the morphology
and function of neural cells and circuits in the intact mammalian brain. However, tissue …

Two-photon calcium imaging provides an optical readout of neuronal activity in populations
of neurons with subcellular resolution. However, conventional two-photon imaging systems …

The understanding of brain computations requires methods that read out neural activity on
different spatial and temporal scales. Following signal propagation and integration across a …

Two-photon microscopy, combined with the appropriate optical labelling, enables the
measurement and tracking of submicrometer structures within brain cells, as well as the …

Optical imaging is important for understanding brain function. However, established
methods with high spatiotemporal resolution are limited by the potential for laser damage to …

Two-photon fluorescence microscopy enables scientists in various fields including
neuroscience,, embryology and oncology to visualize in vivo and ex vivo tissue morphology …

In vivo two-photon calcium imaging would benefit from the use of multiple excitation beams
to increase scanning speed, signal-to-noise ratio and field of view or to image different axial …

High-resolution optical imaging is critical to understanding brain function. We demonstrate
that three-photon microscopy at 1,300-nm excitation enables functional imaging of …

Point-scanning two-photon microscopy enables high-resolution imaging within scattering
specimens such as the mammalian brain, but sequential acquisition of voxels fundamentally …