We imaged transcription in living cells using a locus-specific reporter system, which allowed
precise, single-cell kinetic measurements of promoter binding, initiation and elongation …

Cellular messenger RNA levels are achieved by the combinatorial complexity of factors
controlling transcription, yet the small number of molecules involved in these pathways …

The interaction of transcription factors with target genes is highly dynamic. Whether the
dynamic nature of these interactions is merely an intrinsic property of transcription factors or …

We provide here a molecular movie that captures key aspects of RNA polymerase II initiation
and elongation. To create the movie, we combined structural snapshots of the initiation …

In the eukaryotic genome, the thousands of genes that encode messenger RNA are
transcribed by a molecular machine called RNA polymerase II. Analysing the distribution …

Summary RNA polymerase II (Pol II) transcribes hundreds of kilobases of DNA, limiting the
production of mRNAs and lncRNAs. We used global run-on sequencing (GRO-seq) to …

The regulated transcription of genes determines cell identity and function. Recent structural
studies have elucidated mechanisms that govern the regulation of transcription by RNA …

Forty years of classical biochemical analysis have identified the molecular players involved
in initiation of transcription by eukaryotic RNA polymerase II (Pol II) and largely assigned …

Regulation of gene expression is integral to the development and survival of all organisms.
Transcription begins with the assembly of a pre-initiation complex at the gene promoter …

Quantitative measurement of transcription rates in live cells is important for revealing
mechanisms of transcriptional regulation. This is particularly challenging when measuring …