Cas12f is a hypercompact type V, Cas12 family member. Previously, we reported a set of
engineered guide RNAs supporting high indel efficiency for Cas12f1 in human cells. Here …

Precision genome engineering has dramatically advanced with the development of
CRISPR/Cas base editing systems that include cytosine base editors and adenine base …

Abstract Catalytically inactive CRISPR‐Cas13 (dCas13)‐based base editors can achieve
the conversion of adenine‐to‐inosine (A‐to‐I) or cytidine‐to‐uridine (C‐to‐U) at the RNA …

Although miniature CRISPR-Cas12f systems were recently developed, the editing efficacy
and targeting range of derived miniature cytosine and adenine base editors (miniCBEs and …

Applications of adenine base editors (ABEs) have been constrained by the limited
compatibility of the deoxyadenosine deaminase component with Cas homologs other than …

Compact CRISPR-Cas9 systems that can be packaged into an adeno-associated virus
(AAV) show promise for gene therapy. However, the requirement of protospacer adjacent …

Cytosine base editors (CBEs) efficiently generate precise C· G-to-T· A base conversions, but
the activation-induced cytidine deaminase/apolipoprotein B mRNA-editing enzyme catalytic …

Molecular cloning is an essential technique in molecular biology and biochemistry, but it is
frequently laborious when adequate restriction enzyme recognition sites are absent. Cas9 …

Existing adenine and cytosine base editors induce only a single type of modification, limiting
the range of DNA alterations that can be created. Here we describe a CRISPR–Cas9-based …

As a miniature RNA-guided endonuclease, IscB is presumed to be the ancestor of Cas9 and
to share similar functions. IscB is less than half the size of Cas9 and thus more suitable for in …