Real-time polymerase chain reaction: Current techniques, applications, and role in COVID-19 diagnosis

IM Artika, YP Dewi, IM Nainggolan, JE Siregar… - Genes, 2022 - mdpi.com
Successful detection of the first SARS-CoV-2 cases using the real-time polymerase chain
reaction (real-time PCR) method reflects the power and usefulness of this technique. Real …

Recent advances and challenges of RT-PCR tests for the diagnosis of COVID-19

M Teymouri, S Mollazadeh, H Mortazavi… - … -Research and Practice, 2021 - Elsevier
Since the outbreak of the novel severe acute respiratory syndrome coronavirus-2 (SARS-
CoV-2), the control of virus spread has remained challenging given the pitfalls of the current …

Accuracy of real-time polymerase chain reaction in COVID-19 patients

MJ Lawrence Panchali, HJ Oh, YM Lee… - Microbiology …, 2022 - Am Soc Microbiol
ABSTRACT Coronavirus disease 2019 (COVID-19) is a mild to severe respiratory illness
caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The diagnostic …

Modular micro-PCR system for the onsite rapid diagnosis of COVID-19

PQM Nguyen, M Wang, N Ann Maria, AY Li… - Microsystems & …, 2022 - nature.com
Effective containment of the COVID-19 pandemic requires rapid and accurate detection of
the pathogen. Polymerase chain reaction (PCR) remains the gold standard for COVID-19 …

[PDF][PDF] Fast, portable tests come online to curb coronavirus pandemic

C Sheridan - Nat Biotechnol, 2020 - briancolemd.com
In the rest of the world, most countries have been completely unprepared for the onslaught
of the virus. As a result, many healthcare systems have faced community transmission …

Portable and accurate diagnostics for COVID-19: Combined use of the miniPCR thermocycler and a well-plate reader for SARS-CoV-2 virus detection

E González-González, G Trujillo-de Santiago… - PloS one, 2020 - journals.plos.org
The coronavirus disease 2019 (COVID-19) pandemic has crudely demonstrated the need
for massive and rapid diagnostics. By the first week of July, more than 10,000,000 positive …

Optimization of primer sets and detection protocols for SARS-CoV-2 of coronavirus disease 2019 (COVID-19) using PCR and real-time PCR

M Park, J Won, BY Choi, CJ Lee - Experimental & molecular medicine, 2020 - nature.com
SARS-CoV-2 is very contagious and has rapidly spread globally. Due to various
symptomatic and asymptomatic cases and the possibility of asymptomatic transmission …

[PDF][PDF] Molecular techniques of viral diagnosis

MA Mustafa, MQ AL-Samarraie, MT Ahmed - Science Archives, 2020 - researchgate.net
Viral infections are the cause of very serious problems in humans all over the globe. The
recent outbreak of coronavirus disease 2019 caused by SARS-CoV-2 is a perfect example …

Development and validation of a rapid, single-step reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) system potentially to be used for reliable …

M Jiang, W Pan, A Arasthfer, W Fang, L Ling… - Frontiers in cellular …, 2020 - frontiersin.org
Objectives: Development and validation of a single-step and accurate reverse transcriptase
loop-mediated isothermal amplification technique (RT-LAMP) for rapid identification of …

Applications of digital PCR in COVID‐19 pandemic

C Tan, D Fan, N Wang, F Wang, B Wang, L Zhu, Y Guo - View, 2021 - Wiley Online Library
Abstract The coronavirus disease 2019 (COVID‐19) pandemic has led to a public health
crisis and global panic. This infectious disease is caused by a novel coronavirus named …