Layer-by-layer self-assembly embedding of nattokinase in chitosan/γ-polyglutamic acid: Preparation, fibrinolytic activity, stability, and in vitro digestion study

Z Liu, Y He, H Zhang, X Ma - European Journal of Pharmaceutics and …, 2024 - Elsevier
Nattokinase (NK) is a thrombolytic enzyme extracted from natto, which can be used to
prevent and treat blood clots. However, it is sensitive to the environment, especially the …

Chitosan/casein based microparticles with a bilayer shell–core structure for oral delivery of nattokinase

X Zhang, X Lyu, Y Tong, J Wang, J Ye, R Yang - Food & function, 2020 - pubs.rsc.org
Nattokinase is a thrombolytic enzyme obtained from Japanese traditional food natto for
prevention and cure of thrombosis-related cardiovascular diseases. However, the …

Research progress on the utilisation of embedding technology and suitable delivery systems for improving the bioavailability of nattokinase: a review

X Zhou, L Liu, X Zeng - Food Structure, 2021 - Elsevier
Nattokinase is a thrombolytic drug that is more advantageous than urokinase, streptokinase,
and tissue plasminogen activator because of its low price, high efficiency, and nontoxic side …

Improvement of the stability of nattokinase using γ-polyglutamic acid as a coating material for microencapsulation

CW Hsieh, WC Lu, WC Hsieh, YP Huang… - LWT-Food Science and …, 2009 - Elsevier
In this study, nattokinase (NK) was microencapsulated in high-molecular-weight Na-γ-PGA,
and the amount of NK added to the Na-γ-PGA was in the ratio of 1: 1 and 2: 1 w/w, which …

Preserving the activity of attenuating atherosclerosis of nattokinase in gastrointestinal system by NK/SA/CS microcapsules

M Xie, M Yang, Z Wang, S Cheng, M Du - Food Bioscience, 2024 - Elsevier
The study focused on the role of Nattokinase/Sodium alginate/Chitosan microcapsules
(NK/SA/CS microcapsules) in preserving nattokinase activity in the gastrointestinal system …

Stabilisation and targeted delivery of a fibrinolytic enzyme (nattokinase) by shellac

D Law, Z Zhang - Minerva Biotecnologica, 2007 - search.proquest.com
In this work, the feasibility of using aqueous solution of shellac to encapsulate Nattokinase
based on a simple extrusion method in order to stabilize it in gastric fluid and target its …

Downstream processing, formulation development and antithrombotic evaluation of microbial nattokinase

R Kapoor, H Harde, S Jain, AK Panda… - Journal of Biomedical …, 2015 - ingentaconnect.com
The present research work describes the downstreaming of nattokinase (NK) produced by
Bacillus subtilis under solid state fermentation; and the role of efficient oral formulation of …

Preparation and property studies of enteric-soluble oral nattokinase microcapsules with halloysite nanotubes

W Hu, M Zhou, L Hu, K Huang, S Zhao… - … Journal of Polymeric …, 2024 - Taylor & Francis
Abstract Eudragit L100-55-halloysite nanotubes (EL100-55, HNTs) nanocomposite
microcapsules were introduced to release nattokinase (NK) and avoid the limitations of oral …

Non-covalent modification of thrombolytic agent nattokinase: simultaneous improvement of fibrinolysis activity and enzymatic stability

C Chen, H Duan, C Gao, M Liu, Y Wei, X Zhang, Z Liu - RSC Advances, 2014 - pubs.rsc.org
Cardiovascular disease (CVD) has become the leading cause of death globally. Nattokinase
(NK), as a novel thrombolytic agent, has gained more and more attention. However, NK is …

Protective effect of ovalbumin-flavonoid hydrogel on thrombolytic activity and stability of nattokinase

Y Zhong, L Yang, Z Zhu, H Chen, C Liu, T Dai… - Food Research …, 2022 - Elsevier
The protective effect of ovalbumin-flavonoids (naringenin, genistein, naringin, puerarin, and
daidzein) hydrogels on the thrombolytic activity and stability of nattokinase were …