Potato virus X vector-mediated DNA-free genome editing in plants

H Ariga, S Toki, K Ishibashi - Plant and Cell Physiology, 2020 - academic.oup.com
Genome editing technology is important for plant science and crop breeding. Genome-
edited plants prepared using general CRISPR-Cas9 methods usually contain foreign DNA …

CRISPR-Cas12a genome editing at the whole-plant level using two compatible RNA virus vectors

M Uranga, M Vazquez-Vilar, D Orzáez… - The CRISPR …, 2021 - liebertpub.com
The use of viral vectors that can replicate and move systemically through the host plant to
deliver bacterial CRISPR components enables genome editing at the whole-plant level and …

Efficient virus-mediated genome editing in plants using the CRISPR/Cas9 system

Z Ali, A Abul-Faraj, L Li, N Ghosh, M Piatek, A Mahjoub… - Molecular plant, 2015 - cell.com
Targeted genome editing in plants will not only facilitate functional genomics studies but
also help to discover, expand, and create novel traits of agricultural importance (Pennisi …

Highly efficient DNA-free plant genome editing using virally delivered CRISPR–Cas9

X Ma, X Zhang, H Liu, Z Li - Nature Plants, 2020 - nature.com
Genome-editing technologies using CRISPR–Cas nucleases have revolutionized plant
science and hold enormous promise in crop improvement. Conventional transgene …

Activity and specificity of TRV-mediated gene editing in plants

Z Ali, A Abul-Faraj, M Piatek… - Plant signaling & …, 2015 - Taylor & Francis
Plant trait engineering requires efficient targeted genome-editing technologies. Clustered
regularly interspaced palindromic repeats (CRISPRs)/CRISPR associated (Cas) type II …

Virus-induced plant genome editing

Y Oh, H Kim, SG Kim - Current opinion in plant biology, 2021 - Elsevier
Plant viruses have been engineered to express heterologous proteins and RNAs in plants
for several decades. This viral system can now be applied to editing plant genomes. Virus …

Strategies for delivery of CRISPR/Cas-mediated genome editing to obtain edited plants directly without transgene integration

Z Kocsisova, V Coneva - Frontiers in Genome Editing, 2023 - frontiersin.org
Increased understanding of plant genetics and the development of powerful and easier-to-
use gene editing tools over the past century have revolutionized humankind's ability to …

A simple, flexible and high‐throughput cloning system for plant genome editing via CRISPR‐Cas system

H Kim, ST Kim, J Ryu, MK Choi, J Kweon… - Journal of integrative …, 2016 - Wiley Online Library
CRISPR‐Cas9 system is now widely used to edit a target genome in animals and plants.
Cas9 protein derived from Streptococcus pyogenes (SpCas9) cleaves double‐stranded …

Virus-induced gene editing and its applications in plants

C Zhang, S Liu, X Li, R Zhang, J Li - International Journal of Molecular …, 2022 - mdpi.com
CRISPR/Cas-based genome editing technologies, which allow the precise manipulation of
plant genomes, have revolutionized plant science and enabled the creation of germplasms …

Engineered biocontainable RNA virus vectors for non-transgenic genome editing across crop species and genotypes

Q Liu, C Zhao, K Sun, Y Deng, Z Li - Molecular Plant, 2023 - cell.com
CRISPR/Cas genome-editing tools provide unprecedented opportunities for basic plant
biology research and crop breeding. However, the lack of robust delivery methods has …