Development of an Industrially Feasible Medium for Enhanced Production of Extremely Thermophilic Recombinant Endo-1,4-β-xylanase by Escherichia coli

S Kandiyil, RA Malek, R Aziz, HA El Enshasy - 2018 - nopr.niscpr.res.in
Escherichia coli BL21 (DE3) with a plasmid vector pET-22b (+) carrying xylanase coding
gene isolated from an extremely thermophilic bacterium, Thermotoga neapolitana was used …

A Novel Approach in Lactose Based Induction for Enhanced Production of 1-4-Beta Xylanase by Recombinant Escherichia coli

SK Kandiyil, RA Malek, RH Kaul, CK Ho… - 2019 - nopr.niscpr.res.in
Escherichia coli BL21 (DE3) with a plasmid vector pET-22b (+) carrying xylanase coding
gene isolated from an extremely thermophilic bacterium, Thermotoga sp. was used in this …

[PDF][PDF] Enhanced production of thermophilic xylanase by recombinant Escherichia coli DH5α through optimization of medium and dissolved oxygen level.

B Basar, M Mohd-Shamzi, M Rosfarizan… - 2010 - researchgate.net
Enhancement of thermophilic xylanase production by recombinant Escherichia coli DH5α
through suitable medium formulation was initially investigated using shake flask cultures …

Medium optimization for xylanase production by recombinant Escherichia coli B24

W Rashid, R Abd Malek, MA Wadaan, EA Elsayed… - 2020 - nopr.niscpr.res.in
Interest in xylanase enzyme application has led to production of xylanase from recombinant
Escherichia coli B24, which is an economic alternative towards higher productivity …

Enhanced production of a recombinant xylanase (XT6): Optimization of production and purification, and scaled-up batch fermentation in a stirred tank bioreactor

P Dhaver, T Sithole, B Pletschke, B Sithole… - Scientific Reports, 2023 - nature.com
The endoxylanase XT6 produced by Geobacillus stearothermophilus is a desirable
candidate for industrial applications. In this study, the gene encoding XT6 was cloned using …

Enhanced production of xylanase by recombinant Escherichia coli DH5α through optimization of medium composition using response surface methodology

MR Farliahati, RN Ramanan, R Mohamad… - Annals of …, 2010 - Springer
The effect of medium composition on the production of intracellular xylanase by recombinant
Escherichia coli DH5α was studied in shake flask culture. The medium was optimized using …

Optimization Production of an Endo-β-1,4-Xylanase from Streptomyces thermocarboxydus Using Wheat Bran as Sole Carbon Source

TN Tran, CT Doan, TKL Dinh, THN Duong, TTU Phan… - Recycling, 2024 - mdpi.com
Xylanases, key enzymes for hydrolyzing xylan, have diverse industrial applications. The
bioprocessing of agricultural byproducts to produce xylanase through fermentation …

Gene cloning, expression, immobilization and characterization of endo-xylanase from Geobacillus sp. TF16 and investigation of its industrial applications

U Cakmak, NS Ertunga - Journal of Molecular Catalysis B: Enzymatic, 2016 - Elsevier
The xylanase gene (xynTF16) from a thermophilic bacterium Geobacillus sp. TF16 was
cloned into pET-28a (+) vector and expressed in Escherichia coli BL21 (DE3) pLysS …

Optimal production of a novel endo-acting β-1, 4-xylanase cloned from Saccharophagus degradans 2-40 into Escherichia coli BL21 (DE3)

JK Ko, MW Jung, KH Kim, IG Choi - New biotechnology, 2009 - Elsevier
To date, gene xyn10C from Saccharophagus degradans 2-40 has only been identified to
encode a potential xylanase. In the present study, xyn10C was cloned and overexpressed in …

Efficient xylan-to-sugar biotransformation using an engineered xylanase in hyperthermic environment

C Sha, B Sadaqat, H Wang, X Guo, W Shao - International journal of …, 2020 - Elsevier
Hyperthermophilic xylanases play a critical role in bioconversion from xylan to sugar in the
process of biomass utilization. The discovery of new or improvement of existing xylanases …