Drug affinity responsive target stability (DARTS) is a relatively quick and straightforward
approach to identify potential protein targets for small molecules. It relies on the protection …

Drug affinity responsive target stability (DARTS) is a general methodology for identifying and
studying protein‐ligand interactions. The technique is based on the principle that when a …

Small-molecule target identification is a vital and daunting task for the chemical biology
community as well as for researchers interested in applying the power of chemical genetics …

Drug affinity responsive target stability (DARTS) is a novel target discovery approach and is
particularly adept at screening small molecule (SM) targets without requiring any structural …

Identifying the molecular targets for the beneficial or detrimental effects of small-molecule
drugs is an important and currently unmet challenge. We have developed a method, drug …

We have developed a systematic strategy for drug target identification. This consists of the
following sequential steps:(1) enrichment of total binding proteins using two differential …

Identification and confirmation of bioactive small‐molecule targets is a crucial, often decisive
step both in academic and pharmaceutical research. Through the development and …

Phenotype-based screening has emerged as an alternative route for discovering new
chemical entities toward first-in-class therapeutics. However, clarifying their mode of action …

Identification of the cellular targets of bioactive small molecules is a crucial step in drug
discovery and chemical genetics. Classical affinity purification with small-molecule affinity …

Rapid identification of small molecules that interact with protein targets using a generic
screening method greatly facilitates the development of therapeutic agents. The authors …