The frequently occurring DNA uptake sequence (DUS), recognized as a 10-bp repeat, is
required for efficient genetic transformation in the human pathogens Neisseria meningitidis …

Natural transformation is the main means of horizontal genetic exchange in the obligate
human pathogen Neisseria gonorrhoeae. Neisseria spp. have been shown to preferentially …

In all sexual organisms, adaptations exist that secure the safe reassortment of homologous
alleles and prevent the intrusion of potentially hazardous alien DNA. Some bacteria engage …

Transformation is a complex process that involves several interactions from the binding and
uptake of naked DNA to homologous recombination. Some actions affect transformation …

We created plasmids for use in insertion-duplication mutagenesis (IDM) of Neisseria
gonorrhoeae. This mutagenesis method has the advantage that it requires only a single …

The difficulty in obtaining mutants in pathogenic Neisseria has limited the ability to
genetically define determinants responsible for virulence as well as the ability to generate a …

Repeated sequence signatures are characteristic features of all genomic DNA. We have
made a rigorous search for repeat genomic sequences in the human pathogens Neisseria …

The ability to study the virulence of pathogenic Neisseria spp. has been greatly limited by
the absence of genetic tools which allow the construction of defined mutants. We have …

The chromosome of pathogenic Neisseriae is peppered by members of an abundant family
of small DNA sequences known as Correia elements. These DNA repeats, that we call …

Shuttle mutagenesis using signature-tagged transposons was employed to generate a
library of individually tagged mutants of the Neisseria meningitidis strain B1940, which …