To determine pathways of mRNA turnover in yeast, we have followed the poly (A) tail
removal and degradation of a pulse of newly synthesized transcripts from four different …

The first step in the decay of some eukaryotic mRNAs is the shortening of the poly (A) tail. To
examine how the transcript body was degraded after deadenylation, we followed the decay …

The decay of several yeast mRNAs occurs by a mechanism in which deadenylation
precedes decapping and subsequent 5′-to-3′ exonucleolytic decay. In order to identify …

Decay rates of individual mRNAs in the yeast Saccharomyces cerevisiae can vary by 10-to
20-fold. To determine the basis for the rapid degradation of the mRNA encoded by the yeast …

We developed a procedure to measure mRNA decay rates in the yeast Saccharomyces
cerevisiae and applied it to the determination of half-lives for 20 mRNAs encoded by well …

Publisher Summary This chapter describes the mRNA-destabilizing phenomenon as
nonsense-mediated mRNA decay, and reviews the cis-acting sequences and trans-acting …

The turnover of most mRNAs in Saccharomyces cerevisiae begins with deadenylation
followed by decapping and 5′→ 3′ exonucleolytic digestion. An important question …

In yeast, the major mRNA degradation pathway is initiated by poly (A) tail shortening that
triggers mRNA decapping. The mRNA is then degraded by 5′-to-3′ exonucleolysis. In …

The pathway of mRNA degradation has been extensively studied in the yeast,
Saccharomyces cerevisiae, and it is now clear that many mRNAs decay by a deadenylation …

Several observations in eukaryotic cells suggest that the processes of translation and mRNA
turnover are interrelated. To understand this relationship, we examined the effects of …