Determining the effect of gene deletion is a fundamental approach to understanding gene
function. Conventional genetic screens exhibit biases, and genes contributing to a …

Genome sequencing is leading to the discovery of new genes at a rate 50–100 times greater
than that achieved by classical genetics, but the biological function of almost half of these …

Functional genomic studies in Saccharomyces cerevisiae have contributed enormously to
our understanding of cellular processes. Their full potential, however, has been hampered …

Economical methods by which gene function may be analysed on a genomic scale are
relatively scarce. To fill this need, we have developed a transposon-tagging strategy for the …

A quantitative and highly parallel method for analysing deletion mutants has been
developed to aid in determining the biological function of thousands of newly identified open …

Variation among individuals arises in part from differences in DNA sequences, but the
genetic basis for variation in most traits, including common diseases, remains only partly …

Identifying the mechanisms of eukaryotic genome evolution by comparative genomics is
often complicated by the multiplicity of events that have taken place throughout the history of …

The genome of the yeast Saccharomyces cerevisiae is now completely sequenced1.
Despite successful genetic work in recent years, 60% of yeast genes have no assigned …

Synonymous mutations in protein-coding genes do not alter protein sequences and are thus
generally presumed to be neutral or nearly neutral,,,–. Here, to experimentally verify this …

Genetics aims to understand the relation between genotype and phenotype. However,
because complete deletion of most yeast genes (∼ 80%) has no obvious phenotypic …