Integration Host Factor Binds DNA Holliday Junctions

SH Lin, D Zhao, V Deng, VK Birdsall, S Ho… - International Journal of …, 2022 - mdpi.com
Integration host factor (IHF) is a nucleoid-associated protein involved in DNA packaging,
integration of viral DNA and recombination. IHF binds with nanomolar affinity to duplex DNA …

Single-molecule FRET investigation of binding and the comparative effect of DNA architectural proteins on Holliday junction conformational dynamics

F Islam - Biophysical Journal, 2024 - cell.com
Integration host factor (IHF) of E. coli is a nucleoid-associated protein, pivotal in DNA
packaging, viral DNA integration, and recombination. IHF binds to duplex DNA, forming a 13 …

Unveiling the Holliday junction conformational dynamics upon binding with architectural proteins IHF

F Islam, PP Mishra - bioRxiv, 2023 - biorxiv.org
Integration host factor (IHF) is a vital nucleoid-associated protein that binds strongly to a 13-
base-pair consensus sequence, inducing a significant bend of approximately 160° upon …

Integration host factor bends and bridges DNA in a multiplicity of binding modes with varying specificity

SB Yoshua, GD Watson, JAL Howard… - Nucleic Acids …, 2021 - academic.oup.com
Nucleoid-associated proteins (NAPs) are crucial in organizing prokaryotic DNA and
regulating genes. Vital to these activities are complex nucleoprotein structures, however …

Concerted binding and bending of DNA by Eschericia coli integration host factor

GM Dhavan, DM Crothers, MR Chance… - Journal of molecular …, 2002 - Elsevier
Integration host factor (IHF) is a heterodimeric Eschericia coli protein that plays essential
roles in a variety of cellular processes including site-specific recombination, transcription …

[PDF][PDF] A good turn for DNA: the structure of integration host factor bound to DNA

T Ellenberger, A Landy - Structure, 1997 - cell.com
The crystal structure of integration host factor (IHF) complexed with DNA shows how a small
heterodimeric protein can induce a big bend in DNA. IHF exerts leverage in the minor …

Two-step interrogation then recognition of DNA binding site by Integration Host Factor: an architectural DNA-bending protein

Y Velmurugu, P Vivas, M Connolly… - Nucleic acids …, 2018 - academic.oup.com
The dynamics and mechanism of how site-specific DNA-bending proteins initially interrogate
potential binding sites prior to recognition have remained elusive for most systems. Here we …

[HTML][HTML] Crystal structure of an IHF-DNA complex: a protein-induced DNA U-turn

PA Rice, S Yang, K Mizuuchi, HA Nash - Cell, 1996 - cell.com
Integration host factor (IHF) is a small heterodimeric protein that specifically binds to DNA
and functions as an architectural factor in many cellular processes in prokaryotes. Here, we …

Single base-pair precision and structural rigidity in a small IHF-induced DNA loop

SE Nunes-Düby, LI Smith-Mungo, A Landy - Journal of molecular biology, 1995 - Elsevier
The prokaryotic integration host factor (IHF) is a DNA-binding protein that binds to specific
DNA sites as a heterodimer. Genetic and mutational analyses have previously identified …

Integration host factor: putting a twist on protein–DNA recognition

TW Lynch, EK Read, AN Mattis, JF Gardner… - Journal of molecular …, 2003 - Elsevier
Integration host factor (IHF) is a DNA–bending protein that recognizes its cognate sites
through indirect readout. Previous studies have shown that binding of wild-type (WT)-IHF is …