The two steps of poly (A)-dependent termination, pausing and release, can be uncoupled by truncation of the RNA polymerase II carboxyl-terminal repeat domain
NJ Park, DC Tsao, HG Martinson - Molecular and cellular biology, 2004 - Taylor & Francis
The carboxyl-terminal repeat domain (CTD) of RNA polymerase II is thought to help
coordinate events during RNA metabolism. The mammalian CTD consists of 52 imperfectly …
coordinate events during RNA metabolism. The mammalian CTD consists of 52 imperfectly …
[HTML][HTML] The poly (A) signal, without the assistance of any downstream element, directs RNA polymerase II to pause in vivo and then to release stochastically from the …
IJ Orozco, SJ Kim, HG Martinson - Journal of Biological Chemistry, 2002 - ASBMB
Genes encoding polyadenylated mRNAs depend on their poly (A) signals for termination of
transcription. Typically, transcription downstream of the poly (A) signal gradually declines to …
transcription. Typically, transcription downstream of the poly (A) signal gradually declines to …
[HTML][HTML] Poly (A)-dependent transcription termination: continued communication of the poly (A) signal with the polymerase is required long after extrusion in vivo
SJ Kim, HG Martinson - Journal of Biological Chemistry, 2003 - ASBMB
Genes encoding polyadenylated mRNAs depend on their poly (A) signals for termination of
transcription. An unsolved problem is how the poly (A) signal triggers the polymerase to …
transcription. An unsolved problem is how the poly (A) signal triggers the polymerase to …
The poly (A)-dependent transcriptional pause is mediated by CPSF acting on the body of the polymerase
Eukaryotic poly (A) signals direct mRNA 3′-end processing and also pausing and
termination of transcription. We show that pausing and termination require the processing …
termination of transcription. We show that pausing and termination require the processing …
The conserved AAUAAA hexamer of the poly (A) signal can act alone to trigger a stable decrease in RNA polymerase II transcription velocity
In vivo the poly (A) signal not only directs 3′-end processing but also controls the rate and
extent of transcription. Thus, upon crossing the poly (A) signal RNA polymerase II first …
extent of transcription. Thus, upon crossing the poly (A) signal RNA polymerase II first …
3'RNA processing efficiency plays a primary role in generating termination-competent RNA polymerase II elongation complexes
G Edwalds-Gilbert, J Prescott… - Molecular and cellular …, 1993 - Am Soc Microbiol
In several mammalian transcription units, a transcription termination mechanism in which
efficient termination is dependent on the presence of an intact 3'RNA processing site has …
efficient termination is dependent on the presence of an intact 3'RNA processing site has …
Coordination of RNA polymerase II pausing and 3′ end processing factor recruitment with alternative polyadenylation
B Fusby, S Kim, B Erickson, H Kim… - … and cellular biology, 2016 - Taylor & Francis
Most mammalian genes produce transcripts whose 3′ ends are processed at multiple
alternative positions by cleavage/polyadenylation (CPA). Poly (A) site cleavage frequently …
alternative positions by cleavage/polyadenylation (CPA). Poly (A) site cleavage frequently …
[HTML][HTML] Poly (A) signal-dependent transcription termination occurs through a conformational change mechanism that does not require cleavage at the poly (A) site
Transcription termination for genes encoding polyadenylated mRNAs requires a functional
poly (A) signal (PAS) in the nascent pre-mRNA. Often called PAS-dependent termination, or …
poly (A) signal (PAS) in the nascent pre-mRNA. Often called PAS-dependent termination, or …
Mechanism of poly (A) signal transduction to RNA polymerase II in vitro
DP Tran, SJ Kim, NJ Park, TM Jew… - Molecular and cellular …, 2001 - Am Soc Microbiol
Termination of transcription by RNA polymerase II usually requires the presence of a
functional poly (A) site. How the poly (A) site signals its presence to the polymerase is …
functional poly (A) site. How the poly (A) site signals its presence to the polymerase is …
[HTML][HTML] The mammalian RNA polymerase II C-terminal domain interacts with RNA to suppress transcription-coupled 3′ end formation
RNA polymerase II plays a critical role not only in transcription of mRNA precursors but also
in their subsequent processing. This later function is mediated primarily by the C-terminal …
in their subsequent processing. This later function is mediated primarily by the C-terminal …