Synthesis of magnetic nanoparticles functionalized with histidine and nickel to immobilize His-tagged enzymes using β-galactosidase as a model

BC de Andrade, A Gennari, G Renard… - International Journal of …, 2021 - Elsevier
The aim of this study was to synthesize iron magnetic nanoparticles functionalized with
histidine and nickel (Fe 3 O 4-His-Ni) to be used as support materials for oriented …

Novel and efficient method for immobilization and stabilization of β-d-galactosidase by covalent attachment onto magnetic Fe3O4–chitosan nanoparticles

C Pan, B Hu, W Li, YI Sun, H Ye, X Zeng - Journal of Molecular Catalysis B …, 2009 - Elsevier
A novel and efficient immobilization of β-d-galactosidase from Aspergillus oryzae has been
developed by using magnetic Fe3O4–chitosan (Fe3O4–CS) nanoparticles as support. The …

Immobilization of a β-glucosidase and an endoglucanase in ferromagnetic nanoparticles: A study of synergistic effects

S Carli, LAB de Campos Carneiro, RJ Ward… - Protein Expression and …, 2019 - Elsevier
Nanoparticles can act as support materials for enzymatic immobilization, introducing a
balance of characteristics that modulate the efficiency of biocatalysts, such as specific …

Immobilization of fungal α-galactosidase on magnetic nanoparticles and hydrolysis of raffinose family oligosaccharides (RFO) in soymilk

P Bangoria, S Chaki, AR Shah - Biocatalysis and Biotransformation, 2024 - Taylor & Francis
Immobilization of enzymes is a very attractive technique to develop efficient and cost-
effective bioprocesses. In present investigation, immobilization of partially purified α …

Functionalization of paramagnetic nanoparticles for protein immobilization and purification

LABC Carneiro, RJ Ward - Analytical biochemistry, 2018 - Elsevier
A paramagnetic nanocomposite coated with chitosan and N-(5-Amino-1-carboxy-pentyl)
iminodiacetic acid (NTA) that is suitable for protein immobilization applications has been …

Low-cost, easy-to-prepare magnetic chitosan microparticles for enzymes immobilization

K Pospiskova, I Safarik - Carbohydrate Polymers, 2013 - Elsevier
Extremely simple procedures for the preparation of magnetic chitosan enzyme carriers have
been developed and used for the immobilization of lipase and β-galactosidase as model …

Immobilisation of β-galactosidase onto double layered hydrophilic polymer coated magnetic nanoparticles: Preparation, characterisation and lactose hydrolysis

G Bayramoglu, AG Cimen, MY Arica - International Dairy Journal, 2023 - Elsevier
The surface of polydopamine (PDA) coated magnetic particles was grafted spontaneously
with diaminopolyethylene glycol (DAPEG) as a second polymer layer by conjugation of PDA …

Green construction of recyclable amino-tannic acid modified magnetic nanoparticles: Application for β-glucosidase immobilization

S Moradi, F Khodaiyan, SH Razavi - International journal of biological …, 2020 - Elsevier
The β-glucosidase (BGL) enzyme in food industry is great interest due to its role in food
conversion to produce functional food products. In this study, the BGL was covalently …

Enhancing catalytic performance of β-glucosidase via immobilization on metal ions chelated magnetic nanoparticles

T Chen, W Yang, Y Guo, R Yuan, L Xu, Y Yan - Enzyme and Microbial …, 2014 - Elsevier
A novel magnetic Fe 3 O 4 nanoparticles (MNPs) coupled with agarose (AMNPs) was
synthesized using co-precipitation via alkaline condition and span-80 surfactants in organic …

Immobilization of enzymes on iron oxide magnetic nanoparticles: synthesis, characterization, kinetics and thermodynamics

AB Muley, KH Mulchandani, RS Singhal - Methods in enzymology, 2020 - Elsevier
Immobilization of enzymes on matrices like iron oxide magnetic nanoparticles provides a
new technique against the stagnant conventional approaches for stabilization of enzymes …