The structure-based reaction mechanism of urease, a nickel dependent enzyme: tale of a long debate
This review is an attempt to retrace the chronicle that starts from the discovery of the role of
nickel as the essential metal ion in urease for the enzymatic catalysis of urea, a key step in …
nickel as the essential metal ion in urease for the enzymatic catalysis of urea, a key step in …
Interplay of metal ions and urease
EL Carter, N Flugga, JL Boer, SB Mulrooney… - Metallomics, 2009 - academic.oup.com
Urease, the first enzyme to be crystallized, contains a dinuclear nickel metallocenter that
catalyzes the decomposition of urea to produce ammonia, a reaction of great agricultural …
catalyzes the decomposition of urea to produce ammonia, a reaction of great agricultural …
Structural properties of the nickel ions in urease: novel insights into the catalytic and inhibition mechanisms
This work provides a comprehensive critical summary of urease spectroscopy,
crystallography, inhibitor binding, and site-directed mutagenesis, with special emphasis …
crystallography, inhibitor binding, and site-directed mutagenesis, with special emphasis …
Chemistry of Ni2+ in Urease: Sensing, Trafficking, and Catalysis
Transition metals are both essential to enzymatic catalysis and limited in environmental
availability. These two biological facts have together driven organisms to evolve …
availability. These two biological facts have together driven organisms to evolve …
Structure-based computational study of the catalytic and inhibition mechanisms of urease
The viability of different mechanisms of catalysis and inhibition of the nickel-containing
enzyme urease was explored using the available high-resolution structures of the enzyme …
enzyme urease was explored using the available high-resolution structures of the enzyme …
[HTML][HTML] Ureases: Historical aspects, catalytic, and non-catalytic properties–A review
K Kappaun, AR Piovesan, CR Carlini… - Journal of advanced …, 2018 - Elsevier
Abstract Urease (urea amidohydrolase, EC 3.5. 1.5) is a nickel-containing enzyme produced
by plants, fungi, and bacteria that catalyzes the hydrolysis of urea into ammonia and …
by plants, fungi, and bacteria that catalyzes the hydrolysis of urea into ammonia and …
Nickel enzymes
CT Walsh, WH Orme-Johnson - Biochemistry, 1987 - ACS Publications
Department of Chemistry, Massachusetts Institute of Technology, Cambridge,
Massachusetts 02139 Received April 3, 1987; Revised Manuscript Received May 11, 1987 …
Massachusetts 02139 Received April 3, 1987; Revised Manuscript Received May 11, 1987 …
Metals and their scaffolds to promote difficult enzymatic reactions
SW Ragsdale - Chemical reviews, 2006 - ACS Publications
Approximately one-third of the known enzymes are metalloenzymes. The metal ions
promote a variety of reactions in these enzymes, including bond cleavage and formation …
promote a variety of reactions in these enzymes, including bond cleavage and formation …
Structure-based rationalization of urease inhibition by phosphate: novel insights into the enzyme mechanism
The structure of Bacillus pasteurii urease (BPU) inhibited with phosphate was solved and
refined using synchrotron X-ray diffraction data from a vitrified crystal (1.85 Å resolution …
refined using synchrotron X-ray diffraction data from a vitrified crystal (1.85 Å resolution …
The structure of the elusive urease–urea complex unveils the mechanism of a paradigmatic nickel‐dependent enzyme
Urease, the most efficient enzyme known, contains an essential dinuclear NiII cluster in the
active site. It catalyzes the hydrolysis of urea, inducing a rapid pH increase that has negative …
active site. It catalyzes the hydrolysis of urea, inducing a rapid pH increase that has negative …