Despite numerous viral outbreaks in the last decade, including a devastating global
pandemic, diagnostic and therapeutic technologies remain severely lacking. CRISPR-Cas …

The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-
associated (Cas) systems are RNA-guided sequence-specific prokaryotic antiviral immune …

Abstract Type V and VI CRISPR enzymes are RNA-guided, DNA and RNA-targeting
effectors that allow specific gene knockdown. Cas12 and Cas13 are CRISPR proteins that …

CRISPR‐Cas are adaptable natural prokaryotic defense systems that act against invading
viruses and plasmids. Among the six currently known major CRISPR‐Cas types, the type VI …

RNA-guided binding and cleavage of nucleic acids by CRISPR–Cas systems is a defining
feature of bacterial and archaeal adaptive immunity against viruses and plasmids. As a …

Nucleic acid detection is a necessary part of medical treatment and fieldwork. However, the
current detection technologies are far from ideal. A lack of timely and accessible testing for …

For infectious diseases, rapid and accurate identification of the pathogen is critical for
effective management and treatment, but diagnosis remains challenging, particularly in …

Highlights•Prokaryotic CRISPR/Cas systems confer adaptive immunity against foreign
genetic elements.•Various CRISPR/Cas systems have been adapted for genome …

From its unexpected discovery as a bacterial adaptive immune system to its countless
applications as one of the most versatile gene-editing tools, the CRISPR/Cas9 system has …

Clustered regularly interspaced short palindromic repeats (CRISPR) systems are a set of
versatile gene-editing toolkit that perform diverse revolutionary functions in various fields of …