Molecular and biochemical characterization of the sunflower (Helianthus annuus L.) cytosolic and plastidial enolases in relation to seed development

MA Troncoso-Ponce, J Rivoal, S Dorion, R Sánchez… - Plant science, 2018 - Elsevier
In the present study, we describe the molecular and biochemical characterization of
sunflower (Helianthus annuus L.) enolase (ENO, EC 4.2. 1.11) proteins, which catalyze the …

Isolation of a full-length cDNA encoding cytosolic enolase from Ricinus communis.

SD Blakeley, C Dekroon, KP Cole, M Kraml… - Plant …, 1994 - ncbi.nlm.nih.gov
In glycolysis and gluconeogenesis, enolase (2-phospho-~-glycerate hydrolase, EC 4.2. 1.1
1) is a ubiquitous enzyme that catalyzes the conversion of 2-phosphoglycerate to PEP …

Plant enolase: gene structure, expression, and evolution.

D Van Der Straeten, RA Rodrigues-Pousada… - The Plant …, 1991 - academic.oup.com
Enolase genes were cloned from tomato and Arabidopsis. Comparison of their primary
structures with other enolases revealed a remarkable degree of conservation, except for the …

Purification and differential expression of enolase from maize

SK Lal, PM Kelley, TF Elthon - Physiologia Plantarum, 1994 - Wiley Online Library
Enolase was purified from maze (Zea mays L. inbred B73) seeds to a 55 and 56 kDa protein
doublet based upon sodium dodecyl sulfate–polyacrylamide gel electrophoresis …

[HTML][HTML] Molecular and functional characterization of the plastid-localized phosphoenolpyruvate enolase (ENO1) from Arabidopsis thaliana

V Prabhakar, T Löttgert, T Gigolashvili, K Bell… - FEBS letters, 2009 - Elsevier
The Arabidopsis thaliana gene At1g74030 codes for a putative plastid phosphoenolpyruvate
(PEP) enolase (ENO1). The recombinant ENO1 protein exhibited enolase activity and its …

Characterization of glycolytic initial metabolites and enzyme activities in developing sunflower (Helianthus annuus L.) seeds

MA Troncoso-Ponce, NJ Kruger, G Ratcliffe, R Garcés… - Phytochemistry, 2009 - Elsevier
Unlike other oilseeds (eg Arabidopsis), developing sunflower seeds do not accumulate a lot
of starch and they rely on the sucrose that comes from the mother plant to synthesise lipid …

A Developmental Analysis of the Enolase Isozymes from Ricinus communis

JA Miernyk, DT Dennis - Plant Physiology, 1992 - academic.oup.com
Enolase activity was measured in clarified homogenates of various tissues during the life
cycle of the castor oil plant (Ricinus communis L. cv Baker 296). The proportions of total …

Characterization of a maize cDNA that complements an enolase-deficient mutant of Escherichia coli

SK Lal, S Johnson, T Conway, PM Kelley - Plant Molecular Biology, 1991 - Springer
A cDNA encoding maize enolase (2-phospho-D-glycerate hydrolase) was purified by
functional genetic complementation using an enolase deficient mutant of Escherichia coli …

[PDF][PDF] Molecular cloning and characterization of enolase from oilseed rape (Brassica napus)

JY Zhao, KJ Zuo, J Qin, KX Tang - ACTA BOTANICA SINICA-ENGLISH …, 2004 - Citeseer
An enolase-encoding cDNA clone in oilseed rape (Brassica napus L.) was isolated. This
gene (accession number: AY307449) had a total length of 1 624 bp with an open reading …

Enolase isozymes from developing oilseeds: a survey

JA Miernyk, DT Dennis - Journal of plant physiology, 1987 - Elsevier
Enolase activity was used to demonstrate the occurrence of both plastid and cytosolic
glycolysisin the developing storage organs of several oil-rich seeds. Plastids isolated from …