Programmable cell-free transcriptional switches for antibody detection

A Patino Diaz, S Bracaglia, S Ranallo… - Journal of the …, 2022 - ACS Publications
We report here the development of a cell-free in vitro transcription system for the detection of
specific target antibodies. The approach is based on the use of programmable antigen …

Synthetic antigen-conjugated dna systems for antibody detection and characterization

S Ranallo, S Bracaglia, D Sorrentino, F Ricci - ACS sensors, 2023 - ACS Publications
Antibodies are among the most relevant biomolecular targets for diagnostic and clinical
applications. In this Perspective, we provide a critical overview of recent research efforts …

Antibody activation using DNA‐based logic gates

BMG Janssen, M van Rosmalen… - Angewandte …, 2015 - Wiley Online Library
Oligonucleotide‐based molecular circuits offer the exciting possibility to introduce
autonomous signal processing in biomedicine, synthetic biology, and molecular diagnostics …

Plug-and-play protein biosensors using aptamer-regulated in vitro transcription

H Lee, T Xie, B Kang, X Yu, SW Schaffter… - Nature …, 2024 - nature.com
Molecular biosensors that accurately measure protein concentrations without external
equipment are critical for solving numerous problems in diagnostics and therapeutics …

Engineering biosensors with dual programmable dynamic ranges

B Wei, J Zhang, X Ou, X Lou, F Xia… - Analytical …, 2018 - ACS Publications
Although extensively used in all fields of chemistry, molecular recognition still suffers from a
significant limitation: host–guest binding displays a fixed, hyperbolic dose–response curve …

Programmable, multiplexed DNA circuits supporting clinically relevant, electrochemical antibody detection

S Bracaglia, S Ranallo, KW Plaxco, F Ricci - Acs Sensors, 2021 - ACS Publications
Current health emergencies have highlighted the need to have rapid, sensitive, and
convenient platforms for the detection of specific antibodies. In response, we report here the …

Programmable nucleic acid nanoswitches for the rapid, single-step detection of antibodies in bodily fluids

A Porchetta, R Ippodrino, B Marini… - Journal of the …, 2018 - ACS Publications
Antibody detection plays a pivotal role in the diagnosis of pathogens and monitoring the
success of vaccine immunization. However, current serology techniques require multiple …

A logical molecular circuit for programmable and autonomous regulation of protein activity using DNA aptamer–protein interactions

D Han, Z Zhu, C Wu, L Peng, L Zhou… - Journal of the …, 2012 - ACS Publications
Researchers increasingly envision an important role for artificial biochemical circuits in
biological engineering, much like electrical circuits in electrical engineering. Similar to …

Using antibodies to control DNA-templated chemical reactions

L Baranda Pellejero, M Mahdifar, G Ercolani… - Nature …, 2020 - nature.com
DNA-templated synthesis takes advantage of the programmability of DNA-DNA interactions
to accelerate chemical reactions under diluted conditions upon sequence-specific …

[PDF][PDF] In vitro selection of structure‐switching signaling aptamers

R Nutiu, Y Li - Angewandte Chemie International Edition, 2005 - academia.edu
Signaling aptamers are aptamer probes that couple target binding to fluorescent-signal
generation. These molecular sensors have potential applications in biosensing, proteomics …