[HTML][HTML] Linking neurons to network function and behavior by two-photon holographic optogenetics and volumetric imaging
M Dal Maschio, JC Donovan, TO Helmbrecht, H Baier - Neuron, 2017 - cell.com
We introduce a flexible method for high-resolution interrogation of circuit function, which
combines simultaneous 3D two-photon stimulation of multiple targeted neurons, volumetric …
combines simultaneous 3D two-photon stimulation of multiple targeted neurons, volumetric …
[HTML][HTML] Two-photon bidirectional control and imaging of neuronal excitability with high spatial resolution in vivo
Sensory information is encoded within the brain in distributed spatiotemporal patterns of
neuronal activity. Understanding how these patterns influence behavior requires a method …
neuronal activity. Understanding how these patterns influence behavior requires a method …
Spatially selective holographic photoactivation and functional fluorescence imaging in freely behaving mice with a fiberscope
Correlating patterned neuronal activity to defined animal behaviors is a core goal in
neuroscience. Optogenetics is one large step toward achieving this goal, yet optical …
neuroscience. Optogenetics is one large step toward achieving this goal, yet optical …
Brain-wide circuit interrogation at the cellular level guided by online analysis of neuronal function
Whole-brain imaging allows for comprehensive functional mapping of distributed neural
pathways, but neuronal perturbation experiments are usually limited to targeting predefined …
pathways, but neuronal perturbation experiments are usually limited to targeting predefined …
Submillisecond optogenetic control of neuronal firing with two-photon holographic photoactivation of chronos
Optogenetic neuronal network manipulation promises to unravel a long-standing mystery in
neuroscience: how does microcircuit activity relate causally to behavioral and pathological …
neuroscience: how does microcircuit activity relate causally to behavioral and pathological …
An optogenetic toolbox for unbiased discovery of functionally connected cells in neural circuits
D Förster, M Dal Maschio, E Laurell, H Baier - Nature communications, 2017 - nature.com
Optical imaging approaches have revolutionized our ability to monitor neural network
dynamics, but by themselves are unable to link a neuron's activity to its functional …
dynamics, but by themselves are unable to link a neuron's activity to its functional …
Whole brain functional recordings at cellular resolution in zebrafish larvae with 3D scanning multiphoton microscopy
M Bruzzone, E Chiarello, M Albanesi… - Scientific reports, 2021 - nature.com
Optical recordings of neuronal activity at cellular resolution represent an invaluable tool to
investigate brain mechanisms. Zebrafish larvae is one of the few model organisms where …
investigate brain mechanisms. Zebrafish larvae is one of the few model organisms where …
Simultaneous two-photon imaging and two-photon optogenetics of cortical circuits in three dimensions
The simultaneous imaging and manipulating of neural activity could enable the functional
dissection of neural circuits. Here we have combined two-photon optogenetics with …
dissection of neural circuits. Here we have combined two-photon optogenetics with …
All-optical interrogation of neural circuits in behaving mice
Recent advances combining two-photon calcium imaging and two-photon optogenetics with
computer-generated holography now allow us to read and write the activity of large …
computer-generated holography now allow us to read and write the activity of large …
Holographic imaging and photostimulation of neural activity
Highlights•Computer generated holography can flexibly sculpt light into arbitrary patterns in
3D.•Holograms can mimic most optical transfer functions of a microscope.•Holograms can …
3D.•Holograms can mimic most optical transfer functions of a microscope.•Holograms can …