The pancreatic β-cells sustain systemic glucose homeostasis by producing and secreting
insulin according to the blood glucose levels. Defects in β-cell function are associated with …

Pancreatic beta-cells respond to increasing blood glucose concentrations by secreting the
hormone insulin. The dysfunction of beta-cells leads to hyperglycemia and severe, life …

How pancreatic β-cells acquire function in vivo is a long-standing mystery due to the lack of
technology to visualize β-cell function in living animals. Here, we applied a high-resolution …

Assessing the response of pancreatic islet cells to glucose stimulation is important for
understanding β-cell function. Zebrafish are a promising model for studies of metabolism in …

Visualizing the function of pancreatic β-cells in vivo has been a long-sought goal for β-cell
researchers. Unlike imaging of β-cells in mammalian species with conventional positron …

Intracellular Ca 2+ signaling regulates cellular activities during embryogenesis and in adult
organisms. We generated stable Tg [βactin2: GCaMP6s] stl351 and Tg [ubi: GCaMP6s] …

Coordination of cellular activity through Ca2+ enables β cells to secrete precise quantities of
insulin. To explore how the Ca2+ response is orchestrated in space and time, we implement …

Here, we provide an optimized protocol to observe the interactions between infiltrating
immune cells and islet β cells using live imaging. This protocol is useful for the …

Pancreatic β-cells form highly connected networks within isolated islets. Whether this
behaviour pertains to the situation in vivo, after innervation and during continuous perfusion …

Blood vessel networks provide nutrients and gaseous exchange that are essential for
functions. Pancreatic islet capillaries deliver oxygen to endocrine cells while transporting …