The identification of mutations that are present in a small fraction of DNA templates is
essential for progress in several areas of biomedical research. Although massively parallel …

The identification of mutations that are present in a small fraction of DNA templates is
essential for progress in several areas of biomedical research. Though massively parallel …

Error rates in massively parallel sequencing instruments are generally too high to allow
confident identification of rare variants. An approach that can substantially increase the …

Though massively parallel sequencing instruments are in principle well-suited to the task of
identification of mutations present in a small fraction of DNA templates, the error rates in …

Next-generation DNA sequencing promises to revolutionize clinical medicine and basic
research. However, while this technology has the capacity to generate hundreds of billions …

Identification and quantification of low-frequency mutations remain challenging despite
improvements in the baseline error rate of next-generation sequencing technologies. Here …

With next-generation DNA sequencing technologies, one can interrogate a specific genomic
region of interest at very high depth of coverage and identify less prevalent, rare mutations in …

DNA mutations are the source of genetic variation within populations. The majority of
mutations with observable effects are deleterious. In humans mutations in the germ line can …

Detection of extremely rare variant alleles within a complex mixture of DNA molecules is
becoming increasingly relevant in many areas of clinical and basic research, such as the …

The need in cancer research or evolutionary biology to detect rare mutations or variants
present at very low frequencies (< 10− 5) poses an increasing demand on lowering the …