Optimization of an artificial cellulase cocktail for high-solids enzymatic hydrolysis of cellulosic materials with different pretreatment methods

J Du, J Liang, X Gao, G Liu, Y Qu - Bioresource technology, 2020 - Elsevier
Optimization of the composition of cellulase mixtures is an effective strategy to improve their
hydrolytic efficiency and reduce protein demand during enzymatic degradation of …

Customized optimization of cellulase mixtures for differently pretreated rice straw

IJ Kim, JY Jung, HJ Lee, HS Park, YH Jung… - Bioprocess and …, 2015 - Springer
Lignocellulose contains a large amount of cellulose but is recalcitrant to enzymatic
hydrolysis, which yields sugars for fuels or chemicals. Various pretreatment methods are …

Design of highly efficient cellulase mixtures for enzymatic hydrolysis of cellulose

AV Gusakov, TN Salanovich, AI Antonov… - Biotechnology and …, 2007 - Wiley Online Library
An extremely highly active cellobiohydrolase (CBH IIb or Cel6B) was isolated from
Chrysosporium lucknowense UV18‐25 culture filtrate. The CBH IIb demonstrated the …

Efficiency of new fungal cellulase systems in boosting enzymatic degradation of barley straw lignocellulose

L Rosgaard, S Pedersen, JR Cherry… - Biotechnology …, 2006 - Wiley Online Library
This study examined the cellulytic effects on steam‐pretreated barley straw of cellulose‐
degrading enzyme systems from the five thermophilic fungi Chaetomium thermophilum …

Enzymatic lignocellulose hydrolysis: improved cellulase productivity by insoluble solids recycling

N Weiss, J Börjesson, LS Pedersen, AS Meyer - Biotechnology for biofuels, 2013 - Springer
Background It is necessary to develop efficient methods to produce renewable fuels from
lignocellulosic biomass. One of the main challenges to the industrialization of lignocellulose …

Blending of cellulolytic enzyme preparations from different fungal sources for improved cellulose hydrolysis by increasing synergism

M Adsul, B Sharma, RR Singhania, JK Saini… - RSC Advances, 2014 - pubs.rsc.org
Cellulolytic enzymes were produced from the three fungal strains [P. janthinellum EMS-UV-8
(E), T. reesei Rut C-30 (R) and A. tubingenesis (A)] and used to prepare blends for the …

Additives enhancing enzymatic hydrolysis of lignocellulosic biomass

J Rocha-Martín, C Martinez-Bernal… - Bioresource …, 2017 - Elsevier
Linked to the development of cellulolytic enzyme cocktails from Myceliophthora thermophila,
we studied the effect of different additives on the enzymatic hydrolysis yield. The hydrolysis …

High solids enzymatic hydrolysis of pretreated lignocellulosic materials with a powerful stirrer concept

D Ludwig, B Michael, T Hirth, S Rupp… - Applied biochemistry and …, 2014 - Springer
In this study, we present a powerful stirred tank reactor system that can efficiently hydrolyse
lignocellulosic material at high solid content to produce hydrolysates with glucose …

Practical screening of purified cellobiohydrolases and endoglucanases with α-cellulose and specification of hydrodynamics

G Jäger, Z Wu, K Garschhammer, P Engel… - Biotechnology for …, 2010 - Springer
Background It is important to generate biofuels and society must be weaned from its
dependency on fossil fuels. In order to produce biofuels, lignocellulose is pretreated and the …

Outlook for cellulase improvement: screening and selection strategies

YHP Zhang, ME Himmel, JR Mielenz - Biotechnology advances, 2006 - Elsevier
Cellulose is the most abundant renewable natural biological resource, and the production of
biobased products and bioenergy from less costly renewable lignocellulosic materials is …