Counting cytokinesis proteins globally and locally in fission yeast
JQ Wu, TD Pollard - Science, 2005 - science.org
We used fluorescence microscopy to measure global and local concentrations of 28
cytoskeletal and signaling proteins fused to yellow fluorescent protein (YFP) in the fission …
cytoskeletal and signaling proteins fused to yellow fluorescent protein (YFP) in the fission …
Mitosis in living budding yeast: anaphase A but no metaphase plate
Chromosome movements and spindle dynamics were visualized in living cells of the
budding yeast Saccharomyces cerevisiae. Individual chromosomal loci were detected by …
budding yeast Saccharomyces cerevisiae. Individual chromosomal loci were detected by …
Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor
AF Straight, A Cheung, J Limouze, I Chen… - Science, 2003 - science.org
Completion of cell division during cytokinesis requires temporally and spatially regulated
communication from the microtubule cytoskeleton to the actin cytoskeleton and the cell …
communication from the microtubule cytoskeleton to the actin cytoskeleton and the cell …
Imaging green fluorescent protein fusions in living fission yeast cells
PT Tran, A Paoletti, F Chang - Methods, 2004 - Elsevier
The use of green fluorescent protein (GFP) fusions as biosensors for examining protein
localization and dynamics has revolutionized cell biology. Here, we describe the methods …
localization and dynamics has revolutionized cell biology. Here, we describe the methods …
Spatial and temporal pathway for assembly and constriction of the contractile ring in fission yeast cytokinesis
Microscopy of fluorescent fusion proteins and genetic dependencies show that fission yeast
assemble and constrict a cytokinetic contractile ring in a precisely timed, sequential order …
assemble and constrict a cytokinetic contractile ring in a precisely timed, sequential order …
Ploidy regulation of gene expression
T Galitski, AJ Saldanha, CA Styles, ES Lander, GR Fink - Science, 1999 - science.org
Microarray-based gene expression analysis identified genes showing ploidy-dependent
expression in isogenic Saccharomyces cerevisiae strains that varied in ploidy from haploid …
expression in isogenic Saccharomyces cerevisiae strains that varied in ploidy from haploid …
Spatial organization of cytokinesis signaling reconstituted in a cell-free system
During animal cell division, the cleavage furrow is positioned by microtubules that signal to
the actin cortex at the cell midplane. We developed a cell-free system to recapitulate …
the actin cortex at the cell midplane. We developed a cell-free system to recapitulate …
Chromosome dynamics in the yeast interphase nucleus
Little is known about the dynamics of chromosomes in interphase nuclei. By tagging four
chromosomal regions with a green fluorescent protein fusion to lac repressor, we monitored …
chromosomal regions with a green fluorescent protein fusion to lac repressor, we monitored …
Movement of yeast cortical actin cytoskeleton visualized in vivo.
T Doyle, D Botstein - … of the National Academy of Sciences, 1996 - National Acad Sciences
Fusion proteins between the green fluorescent protein (GFP) and the cytoskeleton proteins
Act1p (actin), Sac6p (yeast fimbrin homolog), and Abp1p in budding yeast (Saccharomyces …
Act1p (actin), Sac6p (yeast fimbrin homolog), and Abp1p in budding yeast (Saccharomyces …
Molecular organization of cytokinesis nodes and contractile rings by super-resolution fluorescence microscopy of live fission yeast
C Laplante, F Huang, IR Tebbs… - Proceedings of the …, 2016 - National Acad Sciences
Cytokinesis in animals, fungi, and amoebas depends on the constriction of a contractile ring
built from a common set of conserved proteins. Many fundamental questions remain about …
built from a common set of conserved proteins. Many fundamental questions remain about …