Stop codon suppression using dedicated tRNA/aminoacyl-tRNA synthetase (aaRS) pairs
allows for genetically encoded, site-specific incorporation of non-canonical amino acids …

Conspectus Expansion of the genetic code allows unnatural amino acids (Uaas) to be site-
specifically incorporated into proteins in live biological systems, thus enabling novel …

The genetic incorporation of non-canonical amino acids (ncAAs) via amber stop codon
suppression is a powerful technique to install artificial probes and reactive moieties onto …

Non-canonical amino acids (ncAAs) can be incorporated into peptides and proteins to
create new properties and functions. Site-specific ncAA incorporation is typically enabled by …

Incorporation of unnatural amino acids (uAAs) via codon reassignment is a powerful
approach for introducing novel chemical and biological properties to synthesized …

The efficient, site-specific introduction of unnatural amino acids into proteins in mammalian
cells is an outstanding challenge in realizing the potential of genetic code expansion …

Genetically encoded unnatural amino acids provide powerful strategies for modulating the
molecular functions of proteins in mammalian cells. However, this approach has not been …

Genetic code expansion enables the incorporation of non-canonical amino acids (ncAAs)
into expressed proteins. ncAAs are usually encoded by a stop codon that is decoded by an …

Engineering protein translation machinery to incorporate noncanonical amino acids (ncAAs)
into proteins has advanced applications ranging from proteomics to single-molecule studies …

The pyrrolysyl-tRNA/pyrrolysyl-tRNA synthetase (PylT/RS) pair from the archaeon
Methanosarcina mazei (Mma) is widely used in protein engineering to site-specifically …