HIGHLIGHTS Generation of a transgenic Cas9-expressing line of tomato cv. Micro-Tom Use
of PVX for sgRNA delivery into Cas9-expressing Micro-Tom plants Heritable proof-of …

Genome editing (GE) using CRISPR/Cas systems has revolutionized plant mutagenesis.
However, conventional transgene-mediated GE methods have limitations due to the time …

Wild tomatoes (Solanum peruvianum) are important genomic resources for tomato research
and breeding. Development of a foreign DNA-free clustered regularly interspaced short …

Current breeding relies mostly on random mutagenesis and recombination to generate
novel genetic variation. However, targeted genome editing is becoming an increasingly …

The narrow genetic base of tomato poses serious challenges in breeding. Hence, with the
advent of clustered regularly interspaced short palindromic repeat (CRISPR)-associated …

CRISPR/Cas9 ribonucleoproteins enable DNA-free genome editing; thus, improving
protoplast culture is essential for the efficient development of mutant plants. However, the …

Crop improvement by inbreeding often results in fitness penalties and loss of genetic
diversity. We introduced desirable traits into four stress-tolerant wild-tomato accessions by …

Viral vectors are being engineered to deliver CRISPR/Cas9 components systemically in
plants to induce somatic or heritable site-specific mutations. It is hypothesized that RNA …

RNA-guided CRISPR/Cas9 technology has been developed for gene/genome editing (GE)
in organisms across kingdoms. However, in planta delivery of the two core GE components …

CRISPR-cas9 mediated gene editing is a powerful tool proven for crop improvement and
has great potential for incorporating novel traits into important genetic resources. In this …