The zebrafish is a valuable vertebrate model to study cardiovascular formation and function
due to the facile visualization and rapid development of the circulatory system in its …

The zebrafish (Danio rerio) has become a popular vertebrate model organism to study organ
formation and function due to its optical clarity and rapid embryonic development. The use of …

In the US and Europe, birth defects are the leading cause of infant mortality. Among birth
defects, Congenital Heart Disease (CHD) occurs in approximately 8 out of 1000 live births …

Zebrafish is a powerful model for forward genetics. Reverse genetic approaches are limited
by the time required to generate stable mutant lines. We describe a system for gene …

Heart disease is the leading cause of death in the United States and worldwide.
Understanding the molecular mechanisms of cardiac development and regeneration will …

The zebrafish has proven to be an excellent organism for manipulation of its genome from a
long history of transcript down-regulation using morpholino oligimers to more recent …

The type II bacterial CRISPR/Cas9 system is rapidly becoming popular for genome-
engineering due to its simplicity, flexibility and high efficiency. Recently, targeted knock-in of …

Congenital malformations are the major cause of infant mortality in the US and Europe. Due
to rapid advances in human genomics, we can now efficiently identify sequence variants that …

A simple and robust method for targeted mutagenesis in zebrafish has long been sought.
Previous methods generate monoallelic mutations in the germ line of F0 animals, usually …

Recent advances with the type II clustered regularly interspaced short palindromic repeats
(CRISPR) system promise an improved approach to genome editing. However, the …