3D mesenchymal stem/stromal cell osteogenesis and autocrine signalling

M Kabiri, B Kul, WB Lott, K Futrega, P Ghanavi… - Biochemical and …, 2012 - Elsevier
Mesenchymal stem/stromal cells (MSC) are rapidly becoming a leading candidate for use in
tissue regeneration, with first generation of therapies being approved for use in orthopaedic …

Turning round: multipotent stromal cells, a three-dimensional revolution?

FA Saleh, PG Genever - Cytotherapy, 2011 - Elsevier
Mesenchymal stromal cells (MSC) can be isolated from adult tissues and induced to
differentiate into skeletal cells, such as osteoblasts, chondrocytes and adipocytes …

Three-dimensional spheroids of mesenchymal stem/stromal cells promote osteogenesis by activating stemness and Wnt/β-catenin

A Imamura, H Kajiya, S Fujisaki, M Maeshiba… - Biochemical and …, 2020 - Elsevier
Mesenchymal stem/stromal cells (MSCs) are multipotent and self-renewal cells that are
widely used in regenerative medicine. The culture of three-dimensional (3D) spheroid MSCs …

[HTML][HTML] 3D spheroid cultivation alters the extent and progression of osteogenic differentiation of mesenchymal stem/stromal cells compared to 2D cultivation

A Wolff, M Frank, S Staehlke, A Springer, O Hahn… - Biomedicines, 2023 - mdpi.com
Mesenchymal stem/stromal cells (MSC) are capable of progenitor cell fraction renewal or
tissue-specific differentiation. These properties are maintained during in vitro cultivation …

[HTML][HTML] Expansion of human mesenchymal stromal cells from fresh bone marrow in a 3D scaffold-based system under direct perfusion

A Papadimitropoulos, E Piccinini, S Brachat, A Braccini… - PloS one, 2014 - journals.plos.org
Mesenchymal stromal/stem cell (MSC) expansion in conventional monolayer culture on
plastic dishes (2D) leads to progressive loss of functionality and thus challenges …

Scaffold-free and scaffold-assisted 3D culture enhances differentiation of bone marrow stromal cells

P Vidyasekar, P Shyamsunder, SK Sahoo… - In Vitro Cellular & …, 2016 - Springer
Abstract 3D cultures of stem cells can preserve differentiation potential or increase the
efficiency of methods that induce differentiation. Mouse bone marrow-derived stromal cells …

Dynamic three-dimensional culture methods enhance mesenchymal stem cell properties and increase therapeutic potential

JE Frith, B Thomson, PG Genever - Tissue Engineering Part C …, 2010 - liebertpub.com
Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation along the
osteogenic, chondrogenic, and adipogenic lineages and have potential applications in a …

[HTML][HTML] Bone marrow-derived stem/stromal cells (BMSC) 3D microtissues cultured in BMP-2 supplemented osteogenic induction medium are prone to adipogenesis

K Futrega, E Mosaad, K Chambers, WB Lott… - Cell and tissue …, 2018 - Springer
Bone marrow-derived mesenchymal stem/stromal cells (BMSC) may facilitate bone repair
through secretion of factors that stimulate endogenous repair processes or through direct …

Three‐dimensional perfusion culture of human bone marrow cells and generation of osteoinductive grafts

A Braccini, D Wendt, C Jaquiery, M Jakob… - Stem …, 2005 - academic.oup.com
Abstract Three‐dimensional (3D) culture systems are critical to investigate cell physiology
and to engineer tissue grafts. In this study, we describe a simple yet innovative bioreactor …

Priming 3D cultures of human mesenchymal stromal cells toward cartilage formation via developmental pathways

M Centola, B Tonnarelli, S Schären… - Stem cells and …, 2013 - liebertpub.com
The field of regenerative medicine has increasingly recognized the importance to be
inspired by developmental processes to identify signaling pathways crucial for 3D …