Enhancing the expression of chondroitin 4-O-sulfotransferase for one-pot enzymatic synthesis of chondroitin sulfate A

W Zhang, P Zhang, H Wang, R Xu, Z Xie, Y Wang… - Carbohydrate …, 2024 - Elsevier
Chondroitin sulfate (CS) stands as a pivotal compound in dietary supplements for
osteoarthritis treatment, propelling significant interest in the biotechnological pursuit of …

Protein engineering, cofactor engineering, and surface display engineering to achieve whole‐cell catalytic production of chondroitin sulfate A

H Liu, W Wei, Z Pang, S Gu, W Song… - Biotechnology and …, 2023 - Wiley Online Library
Chondroitin sulfate A (CSA) is a valuable glycosaminoglycan that has great market demand.
However, current synthetic methods are limited by requiring the expensive sulfate group …

Advances and challenges in biotechnological production of chondroitin sulfate and its oligosaccharides

W Zhang, R Xu, J Chen, H Xiong, Y Wang… - International journal of …, 2023 - Elsevier
Chondroitin sulfate (CS) is a member of glycosaminoglycans (GAGs) and has critical
physiological functions. CS is widely applied in medical and clinical fields. Currently, the …

Enzymatic production of chondroitin oligosaccharides and its sulfate derivatives

W Zhang, R Xu, X Jin, Y Wang, L Hu… - … in Bioengineering and …, 2022 - frontiersin.org
Chondroitin sulfate (CS) has a wide range of physiological functions and clinical
applications. However, the biosynthesis of chondroitin oligosaccharides (o-CHs) and sulfate …

Biosynthesis of animal-free recombinant chondroitin sulfate E using a functional chondroitin sulfotransferase in E. coli

AD Tithi, Y Song, E Paskaleva, M Koffas - Applied Microbiology and …, 2024 - Springer
Abstract Chondroitin sulfate E (CS-E) is a vital sulfated glycosaminoglycan with diverse
biological functions and therapeutic potential. This study marks a significant milestone by …

A microbial–enzymatic strategy for producing chondroitin sulfate glycosaminoglycans

Z Zhou, Q Li, H Huang, H Wang, Y Wang… - Biotechnology and …, 2018 - Wiley Online Library
Chondroitin sulfate has been widely used in both medical and clinical applications.
Commercial chondroitin sulfate has been mainly acquired from animal tissue extraction …

Biosynthesis of non-animal chondroitin sulfate from methanol using genetically engineered Pichia pastoris

X Jin, W Zhang, Y Wang, J Sheng, R Xu, J Li, G Du… - Green …, 2021 - pubs.rsc.org
Chondroitin sulfate (CS) is widely applied in the medical, clinical, and nutraceutical fields.
However, all commercialized CS is extracted from animal tissues, which is associated with …

Design of a biocatalytic cascade for the enzymatic sulfation of unsulfated chondroitin with in situ generation of PAPS

DT Monterrey, R Benito-Arenas, J Revuelta… - … in Bioengineering and …, 2023 - frontiersin.org
Sulfation of molecules in living organisms is a process that plays a key role in their
functionality. In mammals, the sulfation of polysaccharides (glycosaminoglycans) that form …

Construction of a chondroitin sulfate library with defined structures and analysis of molecular interactions

N Sugiura, T Shioiri, M Chiba, T Sato… - Journal of Biological …, 2012 - ASBMB
Chondroitin sulfate (CS) is a linear acidic polysaccharide, composed of repeating
disaccharide units of glucuronic acid and N-acetyl-d-galactosamine and modified with …

[HTML][HTML] Heterologous production of chondroitin

MR Couto, JL Rodrigues, LR Rodrigues - Biotechnology Reports, 2022 - Elsevier
Chondroitin sulfate (CS) is a glycosaminoglycan with a broad range of applications being a
popular dietary supplement for osteoarthritis. Usually, CS is extracted from animal sources …