Quantitative and Multiplex Detection of Extracellular Vesicle‐Derived MicroRNA via Rolling Circle Amplification within Encoded Hydrogel Microparticles

D Al Sulaiman, N Juthani… - Advanced healthcare …, 2022 - Wiley Online Library
Extracellular vesicle‐derived microRNA (EV‐miRNA) represent a promising cancer
biomarker for disease diagnosis and monitoring. However, existing techniques to detect EV …

Extracellular vesicle microRNA quantification from plasma using an integrated microfluidic device

Z Ramshani, C Zhang, K Richards, L Chen… - Communications …, 2019 - nature.com
Extracellular vesicles (EV) containing microRNAs (miRNAs) have tremendous potential as
biomarkers for the early detection of disease. Here, we present a simple and rapid PCR-free …

Two-step competitive hybridization assay: a method for analyzing cancer-related microRNA embedded in extracellular vesicles

S Saha, S Allelein, R Pandey, P Medina-Perez… - Analytical …, 2021 - ACS Publications
With an increased understanding of the role of microRNAs (miRNAs) in cancer evolution,
there is a growing interest in the use of these non-coding nucleic acids in cancer diagnosis …

Encoded hydrogel microparticles for sensitive and multiplex microRNA detection directly from raw cell lysates

H Lee, SJ Shapiro, SC Chapin, PS Doyle - Analytical chemistry, 2016 - ACS Publications
In recent years, microRNAs (miRNAs) have emerged as promising diagnostic markers
because of their unique dysregulation patterns under various disease conditions and high …

An integrated workflow for biomarker development using microRNAs in extracellular vesicles for cancer precision medicine

Y Chen, T Wu, Z Zhu, H Huang, L Zhang, A Goel… - Seminars in Cancer …, 2021 - Elsevier
EV-miRNAs are microRNA (miRNA) molecules encapsulated in extracellular vesicles (EVs),
which play crucial roles in tumor pathogenesis, progression, and metastasis. Recent studies …

Endogenously Gated DNA walking machine for prescreened MicroRNA detection in extracellular vesicles

W Chen, J Sun, Y Mao, Y Tang, J Wang… - Analytical …, 2024 - ACS Publications
Tumor-derived extracellular vesicle (T-EV) microRNAs have been investigated as promising
biomarkers in clinical diagnosis as well as disease progression monitoring. However, the …

DNA Nanowire Guided-Catalyzed Hairpin Assembly Nanoprobe for In Situ Profiling of Circulating Extracellular Vesicle-Associated MicroRNAs

Y Zhang, Y Wu, S Luo, C Yang, G Zhong, G Huang… - ACS …, 2022 - ACS Publications
Extracellular vesicle-associated miRNAs (EV-miRNAs) are emerging as a new type of
noninvasive biomarker for disease diagnosis. Their relatively low abundance, however …

Isolation and quantification of extracellular vesicle-encapsulated microRNA on an integrated microfluidic platform

CY Sung, CC Huang, YS Chen, KF Hsu, GB Lee - Lab on a Chip, 2021 - pubs.rsc.org
Ovarian cancer (OvCa) is the most fatal among gynecological cancers and affects many
women worldwide. Since OvCa is prone to metastasis, which significantly increases …

MicroRNAs in extracellular vesicles: sorting mechanisms, diagnostic value, isolation, and detection technology

D Xu, K Di, B Fan, J Wu, X Gu, Y Sun… - … in Bioengineering and …, 2022 - frontiersin.org
MicroRNAs (miRNAs) are a class of short, single-stranded, noncoding RNAs, with a length
of about 18–22 nucleotides. Extracellular vesicles (EVs) are derived from cells and play a …

Single-step RT-qPCR for detection of extracellular vesicle microRNAs in vivo: A time-and cost-effective method

H Lee, X He, T Le, JM Carnino… - American Journal of …, 2020 - journals.physiology.org
Emerging evidence suggests that extracellular vesicle (EV)-associated microRNAs
(miRNAs) are a potential diagnostic tool for liquid biopsy in various human diseases …