A simple method is described for designing primer sets that can amplify specific protein-
encoding sequences in a wide variety of filamentous ascomycetes. Using this technique, we …

We constructed nine sets of oligonucleotide primers on the basis of the results of DNA
hybridization of cloned genes from Neurospora crassa and Aspergillus nidulans to the …

The validity of reclassifying filamentous, ascomycetous anamorphs solely on the basis of
ribosomal DNA sequences is examined. We suggest that emotional reactions to the debate …

Thorough assessments of fungal diversity are currently hindered by technological
limitations. Here we describe a new method for identifying fungi, oligonucleotide …

Based on morphological and immunological studies, we hypothesize that there are two
lineages within the Sclerotiniaceae, a family of plant-infecting ascomycetes in the order …

In an effort to establish a suitable alternative to the widely used 18S rRNA system for
molecular systematics of fungi, we examined the nuclear gene RPB2, encoding the second …

In our studies of ascomycete evolution we have sought to include representatives of the
largest group of ascomycetes, those 13,500 described species of lichen symbionts. As is …

For the true fungi, phylogenetic relationships inferred from 18S ribosomal DNA sequence
data agree with morphology when (1) the fungi exhibit diagnostic morphological …

Four primer pairs comprising six individual primers were used to amplify regions of β-tubulin
genes from members of the Basidiomycotina. A region of approximately 500 bp, spanning …

DNA sequences were determined for beta tubulin (BT2), calmodulin (CF), ITS and lsu rDNA
(ID) and RNA polymerase II (RPB2) from ca. 460 Aspergillus isolates. RPB2 and rDNA …