We report a computational approach (implemented in MS-DIAL 3.0; http://prime. psc. riken.
jp/) for metabolite structure characterization using fully 13C-labeled and non-labeled plants …

Assignment of individual compound identities within mixtures of thousands of metabolites in
biological extracts is a major challenge for metabolomic technology. Mass spectrometry …

A new strategy for direct infusion-based metabolite analysis employing a combination of
high-resolution mass spectrometry and 13C-isotope labeling of entire metabolomes is …

Annotation of metabolites is an essential, yet problematic, aspect of mass spectrometry (MS)-
based metabolomics assays. The current repertoire of definitive annotations of metabolite …

In this article we discuss current trends in the techniques available for plant metabolomics.
Chemical assignment of unknown metabolites leads to understanding of biosynthetic …

The unbiased and comprehensive analysis of metabolites in any organism presents a major
challenge if proper peak annotation and unambiguous assignment of the biological origin of …

This year marks the 25th anniversary of the coinage of the term metabolome [SG Oliver et
al., Trends Biotech. 16, 373–378 (1998)]. As the field rapidly advances, it is important to take …

Highlights•Methods for stable isotope labeling of plants for metabolomics are
reviewed.•Stable isotope labeling can improve metabolomics annotation and …

Background Calculating the metabolome size of species by genome-guided reconstruction
of metabolic pathways misses all products from orphan genes and from enzymes lacking …

A large number of metabolites are found in each plant, most of which have not yet been
identified. Development of a methodology is required to deal systematically with unknown …