Investigating the impact of vitrification on bovine ovarian tissue morphology, follicle survival, and transcriptomic signature

SP Deligiannis, K Kask, V Modhukur… - Journal of Assisted …, 2024 - Springer
Purpose Ovarian tissue cryopreservation is vital for fertility preservation, yet its effect on
ovarian tissue follicle survival and transcriptomic signature requires further investigation …

P-422 Ovarian tissue vitrification as a low-technology and cost-effective protocol for female fertility preservation: a bovine study

SP Deligiannis, K Kask, V Modhukur… - Human …, 2023 - academic.oup.com
Study question Which are the potential effects of vitrification on ovarian tissue, isolated
follicles and short-term cultured follicles? Summary answer Vitrification-warming protocol …

Cellular and molecular impact of vitrification versus slow freezing on ovarian tissue

E Labrune, P Jaeger, C Santamaria… - … Engineering Part C …, 2020 - liebertpub.com
Objective: To evaluate a vitrification protocol from histology to gene expression to slow
freezing. Methods: Ovaries from 12 prepubertal ewes. The same ovary was cut into …

Damage to fetal bovine ovarian tissue caused by cryoprotectant exposure and vitrification is mitigated during tissue culture

L Mouttham, JE Fortune, P Comizzoli - Journal of assisted reproduction …, 2015 - Springer
Purpose The objective of this study is to characterize the impact of exposure to
cryoprotectants followed by vitrification on primordial follicle survival and activation using a …

Assessment of ovarian tissue and follicular integrity after cryopreservation via slow freezing or vitrification followed by in vitro culture

JI Candelaria, AC Denicol - F&S Science, 2024 - Elsevier
Objective To evaluate ovarian tissue and follicle integrity before and after slow freezing or
vitrification and postthawing in vitro culture. Design A laboratory study using bovine ovarian …

Vitrification leads to transcriptomic modifications of mice ovaries that do not affect folliculogenesis progression

LAAC Pereira, BR Nascimento, EC Jorge… - Reproductive …, 2020 - Elsevier
Ovarian tissue cryopreservation is emerging as a promising alternative for fertility
preservation of cancer survivors. To date, more than a hundred couples have successfully …

Vitrification preserves follicular transcriptomic dynamics during ex vivo ovulation

J Zhang, DD Russo, Y Wang, Q Zhang… - Biology of …, 2023 - academic.oup.com
Vitrification of immature follicles is an emerging approach for studying ovarian biology,
toxicology, fertility preservation, and female contraception. We previously used a 3D …

Human single follicle growth in vitro from cryopreserved ovarian tissue after slow freezing or vitrification

T Wang, J Yan, C Lu, X Xia, T Yin, X Zhi… - Human …, 2016 - academic.oup.com
STUDY QUESTION What is the effect of human ovarian tissue cryopreservation on single
follicular development in vitro? SUMMARY ANSWER Vitrification had a greater negative …

Effect of vitrification on global gene expression dynamics of bovine elongating embryos

E Gutierrez-Castillo, H Ming, B Foster… - Reproduction …, 2021 - CSIRO Publishing
Embryo vitrification involves exposure to high concentrations of cryoprotectants and osmotic
stress during cooling and warming in the cryopreservation process. Many of these factors …

Effect of vitrification on the mRNA transcriptome of bovine oocytes

N Wang, CY Li, HB Zhu, HS Hao… - … in domestic animals, 2017 - Wiley Online Library
Contents Vitrification has been shown to decrease the developmental capacity of
mammalian oocytes, and this is closely associated with the abnormal mRNA expressions of …