Full‐length G glycoprotein directly extracted from rabies virus with detergent and then stabilized by amphipols in liquid and freeze‐dried forms

D Clénet, L Clavier, B Strobbe, C Le Bon… - Biotechnology and …, 2021 - Wiley Online Library
Pathogen surface antigens are at the forefront of the viral strategy when invading host
organisms. These antigens, including membrane proteins (MPs), are broadly targeted by the …

Expression and solubilization of insect cell-based rabies virus glycoprotein and assessment of its immunogenicity and protective efficacy in mice

R Ramya, B Mohana Subramanian… - Clinical and Vaccine …, 2011 - Am Soc Microbiol
Rabies is a fatal zoonotic disease of serious public health and economic significance
worldwide. The rabies virus glycoprotein (RVG) has been the major target for subunit …

[HTML][HTML] Production, purification, and characterization of recombinant rabies virus glycoprotein expressed in PichiaPink™ yeast

H Askri, I Akrouti, S Rourou, H Kallèl - Biotechnology Reports, 2022 - Elsevier
The commonly used host for industrial production of recombinant proteins Pichia pastoris,
has been used in this work to produce the rabies virus glycoprotein (RABV-G). To allow a …

A novel ELISA for quantification of glycoprotein in human rabies vaccines using a clinically proven virus neutralizing human monoclonal antibody

S Gairola, M Gautam, S Waghmare - Human Vaccines & …, 2020 - Taylor & Francis
Global efforts on the replacement of the in vivo rabies vaccine potency test (NIH method)
with in vitro methods for quantification of immunodominant glycoprotein (GP) in rabies …

Expression, purification and immunodetection of a recombinant fragment (residues 179–281) of the G protein from rabies virus ERA strain

ÊJ Bassi, J Vernal, C Zanluca, H Terenzi… - Protein expression and …, 2008 - Elsevier
The glycoprotein (G) of rabies virus (RV) is important for virus infectivity and induction of the
protective immunity. In this study, the region comprising linear epitopes (residues 179–281 …

Immuno-affinity purification of insect cell expressed rabies virus glycoprotein using a conformational specific monoclonal antibody

R Ramya, S Rajalakshmi… - Journal of Advanced …, 2012 - advetresearch.com
Rabies is a disease of nervous system and causes progressive encephalitis with fatal
outcome. The conformation-dependent epitopes on the glycoprotein (G) of rabies virus (RV) …

Demonstration of viral antibodies by pseudotype virus neutralization

E Wright - Current Laboratory Techniques in Rabies Diagnosis …, 2014 - Elsevier
Serological studies of rabies viruses (RABV) using pseudotyped viruses (PV) can be
undertaken in low-containment laboratories, are highly sensitive and specific, and highly …

Purification of a secreted form of recombinant rabies virus glycoprotein: comparison of two affinity tags

BS Wojczyk, M Czerwinski, MM Stwora-Wojczyk… - Protein Expression and …, 1996 - Elsevier
Expression of recombinant eukaryotic proteins in transfected mammalian cell lines has
become an important approach for the characterization of the structure and function of these …

The construction of chimeric rabies virus glycoproteins rescues Arctic-like rabies pseudovirus production

E Bentley, R Ali, DL Horton… - Pseudotype …, 2013 - westminsterresearch.westminster.ac …
Arctic-like rabies viruses (AL RABV) are a lineage of rabies viruses circulating widely in the
Middle East and Asia, with distinct antigenic and genetic characteristics. As with other …

Novel strategy for expression and characterization of rabies virus glycoprotein

R Zhao, Y Shan, M Li, Z Lou, Y Feng, L Huang… - Protein Expression and …, 2020 - Elsevier
Rabies is a fatal zoonosis which could affect all mammals. Glycoprotein (G protein) from the
rabies virus plays an important role in the binding of virus to target cells. However …