Endogenous expression of human membrane type ganglioside sialidase (Neu3) was
examined in various cell lines including NB-1, U87MG, SK-MEL-2, SK-N-MC, HepG2 …

Cytosolic Chinese hamster ovary (CHO) cell sialidase has been cloned as a soluble
glutathione S-transferase (GST)-sialidase fusion protein with an apparent molecular weight …

Gangliosides are plasma membrane components thought to play important roles in cell
surface interactions, cell differentiation, and transmembrane signaling. A mammalian …

Recombinant human cytosolic sialidase (HsNEU2), expressed in Escherichia coli, was
purified to homogeneity, and its substrate specificity was studied. HsNEU2 hydrolyzed 4 …

Several mammalian sialidases have been described so far, suggesting the existence of
numerous polypeptides with different tissue distributions, subcellular localizations and …

Here we report the cDNA sequence of a human ganglioside sialidase. The cDNA was
isolated from a human brain cDNA library by screening with a 240 bp probe generated by …

Sialidases are glycosidases responsible for the removal of α-glycosidically linked sialic acid
residues from carbohydrate portions of glycoproteins and glycolipids, this process being the …

A gangliosidespecific sialidase that controls cellular functions such as growth, differentiation,
and adhesion has been observed in a variety of cells, but its characterization proved difficult …

The cDNA of Chinese hamster ovary (CHO) cell cytosolic sialidase was amplified by RT-
PCR and cloned into the pGEX-2T plasmid vector encoding for glutathione S-transferase …

Gangliosides, constituents of surfaces of vertebrate cells, modulate important cellular
functions. Ganglioside‐specific sialidases that possibly control these processes have been …