Background: Combined analysis of DNA content and immunofluorescence on single cells by
flow cytometry provides information on the proliferative response of subpopulations to stimuli …

Combined analysis of DNA content and immunofluorescence on single cells by flow
cytometry provides information on the proliferative response of cellular sub-populations in …

Combined analysis of single cell DNA content and immunofluorescence by flow cytometry is
complementary to tritiated thymidine analysis of cellular proliferation, allowing detailed …

BACKGROUND Internal standards have been used in flow cytometry methods to enumerate
lymphoid subsets and hemopoietic progenitor cells ex vivo. However, the currently available …

Background Current methods for multiparameter DNA flow cytometry suffer from several
limitations. These include significant modifications of cell morphological parameters, the …

Dead cells represent a significant source of interference in the flow cytometric analysis of
viable cells primarily due to nonspecific uptake of probes, increased autofluorescence, and …

Many research projects in cell biology now use flow cytometry for analysis or for isolation of
specific cell types. In such studies, cell viability is obviously a crucial issue. However, many …

Publisher Summary Cells that have lost plasma membrane integrity and become permeable
to external compounds, including dyes and enzymes, are considered to be nonviable and …

Flow cytometry is most often used in the clinical laboratory for the purpose of
immunophenotyping. Here, fluorescently labeled antibodies are bound to cell surface …

A vital, nucleic acid stain (LDS‐751) was used to discriminate intact from damaged cells in a
flow cytometer even after the samples had been fixed with paraformaldehyde. Three major …