Purpose: To validate the more easily applicable “flush” tear collection technique as a viable
alternative to basal and reflex tear collection. Materials and Methods: Total protein content …

A protocol for quantitative assay of several proteins in small tear volumes is described.
Assays included sandwich ELISA's for IgM, IgA, secretory IgA, IgG, lactoferrin and “serum” …

Purpose To determine the repeatability of the flush tear collection technique and the
Schirmer strip for Substance P tear analysis. Methods The tears of 10 healthy non–contact …

Tear fluid is emerging as a source of non-invasive biomarkers, both for ocular and systemic
conditions. Accurate quantification of tear proteins can be improved by standardizing …

Background Quantitative determination of tear proteins is critical to our understanding of
those ocular diseases with tear protein changes, but remains technically complex due to the …

PURPOSE. Tear protein composition alters during eye closure, by becoming rich in
secretory IgA (sIgA) and certain complement proteins. This may reflect altered ocular …

Purpose Ocular discomfort is among the main causes of contact lens wear discontinuation.
This study investigated the association between subjective ocular comfort ratings and …

PURPOSE. To determine the effect of storage on protein concentration of tear samples
stored at room temperature (RT), 4° C,-220° C and-70° C. METHODS. Total protein …

Purpose. To compare the amount of protein deposits adhering to human-worn soft contact
lenses, using the untreated contact lens from the contralateral eye as controls, to investigate …

Purpose.: Low tear volume limits the use of nonstimulated (NS) microcapillary tear collection
in aqueous-deficient (AD) patients. Adding a small amount of “washout” fluid to the eye prior …