The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes

R Silva-Rocha, E Martínez-García, B Calles… - Nucleic acids …, 2013 - academic.oup.com
Abstract The 'Standard European Vector Architecture'database (SEVA-DB, http://seva. cnb.
csic. es) was conceived as a user-friendly, web-based resource and a material clone …

SEVA 3.0: an update of the Standard European Vector Architecture for enabling portability of genetic constructs among diverse bacterial hosts

E Martínez-García, A Goni-Moreno… - Nucleic acids …, 2020 - academic.oup.com
Abstract The Standard European Vector Architecture 3.0 database (SEVA-DB 3.0,
http://seva. cnb. csic. es) is the update of the platform launched in 2013 both as a web-based …

SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes

E Martínez-García, S Fraile, E Algar… - Nucleic Acids …, 2023 - academic.oup.com
Abstract The SEVA platform (https://seva-plasmids. com) was launched one decade ago,
both as a database (DB) and as a physical repository of plasmid vectors for genetic analysis …

SEVA 2.0: an update of the Standard European Vector Architecture for de-/re-construction of bacterial functionalities

E Martínez-García, T Aparicio… - Nucleic acids …, 2015 - academic.oup.com
Abstract The Standard European Vector Architecture 2.0 database (SEVA-DB 2.0,
http://seva. cnb. csic. es) is an improved and expanded version of the platform released in …

The art of vector engineering: towards the construction of next‐generation genetic tools

LC Nora, CA Westmann… - Microbial …, 2019 - Wiley Online Library
When recombinant DNA technology was developed more than 40 years ago, no one could
have imagined the impact it would have on both society and the scientific community. In the …

SEVA 3.1: enabling interoperability of DNA assembly among the SEVA, BioBricks and Type IIS restriction enzyme standards

SG Damalas, C Batianis… - Microbial …, 2020 - Wiley Online Library
Robust synthetic biology applications rely heavily on the design and assembly of DNA parts
with specific functionalities based on engineering principles. However, the assembly …

pBAM1: an all-synthetic genetic tool for analysis and construction of complex bacterial phenotypes

E Martínez-García, B Calles, M Arévalo-Rodríguez… - BMC microbiology, 2011 - Springer
Background Since publication in 1977 of plasmid pBR322, many breakthroughs in Biology
have depended on increasingly sophisticated vector platforms for analysis and engineering …

Alliance of Genome Resources Portal: unified model organism research platform

Nucleic acids research, 2020 - academic.oup.com
Abstract The Alliance of Genome Resources (Alliance) is a consortium of the major model
organism databases and the Gene Ontology that is guided by the vision of facilitating …

Unknown unknowns: essential genes in quest for function

A Danchin, G Fang - Microbial Biotechnology, 2016 - Wiley Online Library
The experimental design of a minimal synthetic genome revealed the presence of a large
number of genes without ascribed function, in part because the abstract laws of life must be …

Diversity, versatility and complexity of bacterial gene regulation mechanisms: opportunities and drawbacks for applications in synthetic biology

I Bervoets, D Charlier - FEMS microbiology reviews, 2019 - academic.oup.com
Gene expression occurs in two essential steps: transcription and translation. In bacteria, the
two processes are tightly coupled in time and space, and highly regulated. Tight regulation …