Tissue storage and primer selection influence pyrosequencing‐based inferences of diversity and community composition of endolichenic and endophytic fungi
JM U'Ren, JM Riddle, JT Monacell… - Molecular Ecology …, 2014 - Wiley Online Library
Next‐generation sequencing technologies have provided unprecedented insights into
fungal diversity and ecology. However, intrinsic biases and insufficient quality control in next …
fungal diversity and ecology. However, intrinsic biases and insufficient quality control in next …
[HTML][HTML] ITS2 metabarcoding analysis complements lichen mycobiome diversity data
Lichen thalli harbor complex fungal communities (mycobiomes) of species with divergent
trophic and ecological strategies. The complexity and diversity of lichen mycobiomes are still …
trophic and ecological strategies. The complexity and diversity of lichen mycobiomes are still …
ITS 1 metabarcoding highlights low specificity of lichen mycobiomes at a local scale
F Fernández‐Mendoza, A Fleischhacker… - Molecular …, 2017 - Wiley Online Library
As self‐supporting and long‐living symbiotic structures, lichens provide a habitat for many
other organisms beside the traditionally considered lichen symbionts—the myco‐and the …
other organisms beside the traditionally considered lichen symbionts—the myco‐and the …
Is targeted community DNA metabarcoding suitable for biodiversity inventories of lichen-forming fungi?
B Wright, LLS Clair, SD Leavitt - Ecological Indicators, 2019 - Elsevier
Comprehensive lichen inventories at biomonitoring reference sites provide valuable
ecological information but are notoriously difficult to obtain. Due to the limited number of …
ecological information but are notoriously difficult to obtain. Due to the limited number of …
Endolichenic Fungal Community Analysis by Pure Culture Isolation and Metabarcoding: A Case Study of Parmotrema tinctorum
Lichen is a symbiotic mutualism of mycobiont and photobiont that harbors diverse organisms
including endolichenic fungi (ELF). Despite the taxonomic and ecological significance of …
including endolichenic fungi (ELF). Despite the taxonomic and ecological significance of …
Barcoding lichen-forming fungi using 454 pyrosequencing is challenged by artifactual and biological sequence variation
K Mark, C Cornejo, C Keller, D Flück, C Scheidegger - Genome, 2016 - cdnsciencepub.com
Although lichens (lichen-forming fungi) play an important role in the ecological integrity of
many vulnerable landscapes, only a minority of lichen-forming fungi have been barcoded …
many vulnerable landscapes, only a minority of lichen-forming fungi have been barcoded …
Fungal community analysis by high‐throughput sequencing of amplified markers–a user's guide
BD Lindahl, RH Nilsson, L Tedersoo… - New …, 2013 - Wiley Online Library
Novel high‐throughput sequencing methods outperform earlier approaches in terms of
resolution and magnitude. They enable identification and relative quantification of …
resolution and magnitude. They enable identification and relative quantification of …
Meta‐analysis of deep‐sequenced fungal communities indicates limited taxon sharing between studies and the presence of biogeographic patterns
High‐throughput amplicon sequencing gives new insights into fungal community ecology.
Massively generated molecular data lead to the discovery of vast fungal diversity. However …
Massively generated molecular data lead to the discovery of vast fungal diversity. However …
Quantifying microbial communities with 454 pyrosequencing: does read abundance count?
Pyrosequencing technologies have revolutionized how we describe and compare complex
microbial communities. In 454 pyrosequencing data sets, the abundance of reads pertaining …
microbial communities. In 454 pyrosequencing data sets, the abundance of reads pertaining …
Comparison of ITS1 and ITS2 rDNA in 454 sequencing of hyperdiverse fungal communities
Next-generation amplicon sequencing is a powerful tool in ecological studies of fungi.
Technological development suggests that short fragment high-throughput techniques, eg …
Technological development suggests that short fragment high-throughput techniques, eg …