Tissue storage and primer selection influence pyrosequencing‐based inferences of diversity and community composition of endolichenic and endophytic fungi

JM U'Ren, JM Riddle, JT Monacell… - Molecular Ecology …, 2014 - Wiley Online Library
Next‐generation sequencing technologies have provided unprecedented insights into
fungal diversity and ecology. However, intrinsic biases and insufficient quality control in next …

[HTML][HTML] ITS2 metabarcoding analysis complements lichen mycobiome diversity data

E Banchi, D Stankovic, F Fernández-Mendoza… - Mycological …, 2018 - Springer
Lichen thalli harbor complex fungal communities (mycobiomes) of species with divergent
trophic and ecological strategies. The complexity and diversity of lichen mycobiomes are still …

ITS 1 metabarcoding highlights low specificity of lichen mycobiomes at a local scale

F Fernández‐Mendoza, A Fleischhacker… - Molecular …, 2017 - Wiley Online Library
As self‐supporting and long‐living symbiotic structures, lichens provide a habitat for many
other organisms beside the traditionally considered lichen symbionts—the myco‐and the …

Is targeted community DNA metabarcoding suitable for biodiversity inventories of lichen-forming fungi?

B Wright, LLS Clair, SD Leavitt - Ecological Indicators, 2019 - Elsevier
Comprehensive lichen inventories at biomonitoring reference sites provide valuable
ecological information but are notoriously difficult to obtain. Due to the limited number of …

Endolichenic Fungal Community Analysis by Pure Culture Isolation and Metabarcoding: A Case Study of Parmotrema tinctorum

JH Yang, SY Oh, W Kim, JS Hur - Mycobiology, 2022 - Taylor & Francis
Lichen is a symbiotic mutualism of mycobiont and photobiont that harbors diverse organisms
including endolichenic fungi (ELF). Despite the taxonomic and ecological significance of …

Barcoding lichen-forming fungi using 454 pyrosequencing is challenged by artifactual and biological sequence variation

K Mark, C Cornejo, C Keller, D Flück, C Scheidegger - Genome, 2016 - cdnsciencepub.com
Although lichens (lichen-forming fungi) play an important role in the ecological integrity of
many vulnerable landscapes, only a minority of lichen-forming fungi have been barcoded …

Fungal community analysis by high‐throughput sequencing of amplified markers–a user's guide

BD Lindahl, RH Nilsson, L Tedersoo… - New …, 2013 - Wiley Online Library
Novel high‐throughput sequencing methods outperform earlier approaches in terms of
resolution and magnitude. They enable identification and relative quantification of …

Meta‐analysis of deep‐sequenced fungal communities indicates limited taxon sharing between studies and the presence of biogeographic patterns

A Meiser, M Bálint, I Schmitt - New Phytologist, 2014 - Wiley Online Library
High‐throughput amplicon sequencing gives new insights into fungal community ecology.
Massively generated molecular data lead to the discovery of vast fungal diversity. However …

Quantifying microbial communities with 454 pyrosequencing: does read abundance count?

AS Amend, KA Seifert, TD Bruns - Molecular ecology, 2010 - Wiley Online Library
Pyrosequencing technologies have revolutionized how we describe and compare complex
microbial communities. In 454 pyrosequencing data sets, the abundance of reads pertaining …

Comparison of ITS1 and ITS2 rDNA in 454 sequencing of hyperdiverse fungal communities

AL Bazzicalupo, M Balint, I Schmitt - Fungal Ecology, 2013 - Elsevier
Next-generation amplicon sequencing is a powerful tool in ecological studies of fungi.
Technological development suggests that short fragment high-throughput techniques, eg …