A preliminary study of the use of MinION sequencing to specifically detect Shiga toxin-producing Escherichia coli in culture swipes containing multiple serovars of this …

HS Tunsjø, IF Ullmann, C Charnock - Scientific Reports, 2023 - nature.com
An important challenge relating to clinical diagnostics of the foodborne pathogen Shiga toxin-
producing E. coli (STEC), is that PCR-detection of the shiga-toxin gene (stx) in DNA from …

Inconsistent PCR detection of Shiga toxin-producing Escherichia coli: Insights from whole genome sequence analyses

VS Castro, R Ortega Polo, EES Figueiredo… - PLoS …, 2021 - journals.plos.org
Shiga toxin-producing Escherichia coli (STEC) have been linked to food-borne disease
outbreaks. As PCR is routinely used to screen foods for STEC, it is important that factors …

Whole-genome single-nucleotide polymorphism (SNP) analysis applied directly to stool for genotyping shiga toxin-producing Escherichia coli: an advanced molecular …

N Singh, P Lapierre, TM Quinlan… - Journal of Clinical …, 2019 - Am Soc Microbiol
Whole-genome sequencing (WGS) of pathogens from pure culture provides unparalleled
accuracy and comprehensive results at a cost that is advantageous compared with …

Identification of Novel Biomarkers for Priority Serotypes of Shiga Toxin-Producing Escherichia coli and the Development of Multiplex PCR for Their Detection

M Kiel, P Sagory-Zalkind, C Miganeh, C Stork… - Frontiers in …, 2018 - frontiersin.org
It would be desirable to have an unambiguous scheme for the typing of Shiga toxin-
producing Escherichia coli (STEC) isolates to subpopulations. Such a scheme should take …

Isolation and detection of Shiga toxin-producing Escherichia coli in clinical stool samples using conventional and molecular methods

MW Gilmour, L Chui, T Chiu… - Journal of medical …, 2009 - microbiologyresearch.org
The isolation of Shiga toxin-producing Escherichia coli (STEC) other than serogroup O157
from clinical stool samples is problematic due to the lack of differential phenotypic …

[HTML][HTML] Whole genome sequencing characterization of Shiga toxin–producing Escherichia coli isolated from flour from Swiss retail markets

R Boss, J Hummerjohann - Journal of food protection, 2019 - Elsevier
Shiga toxin–producing Escherichia coli (STEC) strains are often found in food and cause
human infections. Although STEC O157: H7 is most often responsible for human disease …

Exploring Long-Read Metagenomics for Full Characterization of Shiga Toxin-Producing Escherichia coli in Presence of Commensal E. coli

S Jaudou, C Deneke, ML Tran, C Salzinger, F Vorimore… - Microorganisms, 2023 - mdpi.com
The characterization of Shiga toxin-producing Escherichia coli (STEC) is necessary to
assess their pathogenic potential, but isolation of the strain from complex matrices such as …

A multiple protocol to improve diagnosis and isolation of Shiga toxin-producing Escherichia coli from human stool specimens

DR Shelton, JS Karns, CH Park - Diagnostic microbiology and infectious …, 2008 - Elsevier
Many infections caused by Shiga toxin-producing Escherichia coli (STEC) are undiagnosed,
particularly non-O157 STEC. We evaluated the use of a multiple protocol approach to …

Development of 11-Plex MOL-PCR Assay for the Rapid Screening of Samples for Shiga Toxin-Producing Escherichia coli

TA Woods, HM Mendez, S Ortega, X Shi… - Frontiers in Cellular …, 2016 - frontiersin.org
Strains of Shiga toxin-producing Escherichia coli (STEC) are a serious threat to the health,
with approximately half of the STEC related food-borne illnesses attributable to …

Rapid Identification and Differentiation of Non-O157 Shiga Toxin–Producing Escherichia coli Using Polymerase Chain Reaction Coupled to Electrospray Ionization …

J Shen, F Wang, F Li, R Housley, H Carolan… - Foodborne …, 2013 - liebertpub.com
A polymerase chain reaction (PCR)–mass spectroscopy assay was developed to identify
non-O157 Shiga toxin–producing Escherichia coli (STEC) with Plex-ID biosensor system, a …