In industrial bioprocesses, microbial metabolism dictates the product yields, and therefore,
our capacity to control it has an enormous potential to help us move towards a bio-based …

Metabolic engineering aims to develop efficient cell factories by rewiring cellular
metabolism. As one of the most commonly used cell factories, Saccharomyces cerevisiae …

Highlights•Non-model microorganisms serve as promising industrial chassis for
bioproduction.•CRISPR-based genetic modifications enable desired production …

CRISPR/Cas9 is a simple and efficient tool for targeted and marker-free genome
engineering. Here, we report the development and successful application of a multiplex …

A highly effective metabolic pathway is the key for an efficient cell factory. However, the
engineered homologous or heterologous multi-gene pathway may be unbalanced …

The application of rational design in reallocating metabolic flux to overproduce desired
chemicals is always restricted by the native regulatory network. Here, we demonstrated that …

Optimization of metabolic flux is a difficult and time‐consuming process that often involves
changing the expression levels of multiple genes simultaneously. While some pathways …

Highlights•Bacterial cell factory development requires highly efficient metabolic
engineering.•CRISPR-Cas-based engineering accelerates industrial strain …

Highlights•CRISPR activation is an emerging tool for engineering bacterial metabolism.•The
promoter design rules for effective CRISPR activation in bacteria are complex.•Guide RNAs …

Engineered metabolic pathways can be augmented with dynamic regulatory controllers to
increase production titers by minimizing toxicity and helping cells maintain homeostasis. We …