Effects of fetal calf serum, amino acids, vitamins and insulin on blastocoel formation and hatching of in vivo and IVM/IVF-derived porcine embryos developing in vitro

DB Koo, NH Kim, HT Lee, KS Chung - Theriogenology, 1997 - Elsevier
The objective of this study was to determine the effects of fetal calf serum (FCS), non-
essential MEM amino acids, MEM vitamins and insulin on blastocoel formation, expansion …

Development and viability of in vitro derived porcine blastocysts cultured in NCSU23 and G1. 2/G2. 2 sequential medium

JE Swain, CL Bormann, RL Krisher - Theriogenology, 2001 - Elsevier
Porcine embryo development in vitro is relatively inefficient compared to other domestic
species. Currently, a single culture medium (NCSU23) is the standard for porcine in vitro …

In vitro development to blastocysts of early porcine embryos produced in vivo or in vitro

D Rath, H Niemann, CRL Torres - Theriogenology, 1995 - Elsevier
The objective of this study was to compare the development of porcine embryos from the 2-
and 4-cell stages to the blastocyst stage after in vivo or in vitro fertilization and in vivo or in …

Development and quality of porcine embryos in different culture system and embryo-producing methods

SA Ock, SL Lee, JG Kim, BM Kumar… - Zygote, 2007 - cambridge.org
In this study, the developmental ability and cellular composition of porcine IVF, parthenote
and somatic cell nuclear transfer (SCNT) embryos were evaluated following different in vitro …

Improved in vitro development of porcine embryos with different energy substrates and serum

H Kim, G Lee, S Hyun, S Lee, D Nam, Y Jeong, S Kim… - Theriogenology, 2004 - Elsevier
The effect of replacing 5.5 mM glucose in North Carolina State University (NCSU)-23
medium with 0.5 mM pyruvate/5.0 mM lactate on porcine IVF embryo development was …

In vitro development following one-step dilution of OPS-vitrified porcine blastocysts

C Cuello, MA Gil, I Parrilla, J Tornel, JM Vázquez… - Theriogenology, 2004 - Elsevier
The objective of this experiment was to compare the in vitro survival and hatching rates of
OPS-vitrified porcine blastocysts obtained after conventional (three-step dilution) or direct …

Defined system for in vitro production of porcine embryos using a single basic medium

K Yoshioka, C Suzuki, A Onishi - Journal of Reproduction and …, 2008 - jstage.jst.go.jp
We have previously indicated that porcine blastocysts can be produced by in vitro
fertilization (IVF) and culture (IVC) in chemically defined porcine gamete medium (PGM) and …

Effect of replacement of pyruvate/lactate in culture medium with glucose on preimplantation development of porcine embryos in vitro

NWK Karja, S Medvedev, A Onishi… - … of Reproduction and …, 2004 - jstage.jst.go.jp
The effect of glucose supplementation at different times in in vitro culture on the
developmental competence of in vitro produced (IVP) porcine embryos was examined. In …

Effect of maturation media and oocytes derived from sows or gilts on the development of cloned pig embryos

SH Hyun, GS Lee, DY Kim, HS Kim, SH Lee, S Kim… - Theriogenology, 2003 - Elsevier
In order to develop a culture system and recipient cytoplasm that could improve the
developmental competence of somatic cell nuclear transfer (SCNT) embryos for successful …

The quality after culture in vitro or in vivo of porcine oocytes matured and fertilized in vitro and their ability to develop to term

Y Nakamura, S Tajima, K Kikuchi - Animal Science Journal, 2017 - Wiley Online Library
The quality of porcine blastocysts produced in vitro is poor in comparison with those that
develop in vivo. We examined the quality of in vitro‐matured and fertilized (IVM/IVF) oocytes …