Production of soluble truncated spike protein of porcine epidemic diarrhea virus from inclusion bodies of Escherichia coli through refolding

DC Piao, YS Lee, JD Bok, CS Cho, ZS Hong… - Protein expression and …, 2016 - Elsevier
The emergence of highly pathogenic variant porcine epidemic diarrhea virus (PEDV) strains,
from 2013 to 2014, in North American and Asian countries have greatly threatened global …

Optimization of expression and purification of recombinant S1 domain of the porcine epidemic diarrhea virus spike (PEDV- S1) protein in Escherichia coli

N Van Noi, YC Chung - Biotechnology & Biotechnological …, 2017 - Taylor & Francis
The PEDV-S1 protein, one of the members of a large family of PEDV, is significantly
upregulated and has been targeted as a biomarker of cellular stress in several studies …

[HTML][HTML] Identification of a novel B-cell epitope in the spike protein of porcine epidemic diarrhea virus

N Kong, Q Meng, Y Jiao, Y Wu, Y Zuo, H Wang, D Sun… - Virology journal, 2020 - Springer
Background Porcine epidemic diarrhea virus (PEDV) infection causes an acute enteric tract
infectious disease characterized by vomiting, anorexia, dehydration, weight loss and high …

[HTML][HTML] Immunogenicity and protective efficacy of recombinant S1 domain of the porcine epidemic diarrhea virus spike protein

J Oh, KW Lee, HW Choi, C Lee - Archives of virology, 2014 - Springer
Porcine epidemic diarrhea virus (PEDV) is a highly contagious enteric pathogen of swine.
Acute PEDV outbreaks have continually emerged in most swine-producing Asian countries …

Generation of a monoclonal antibody to S1 protein of porcine epidemic diarrhea virus

L Cao, Z Qin, X Ge, X Yin, C Xia, R Bu… - Monoclonal …, 2013 - liebertpub.com
Gene encoding the N-terminal half of spike protein (S1) of porcine epidemic diarrhea virus
(PEDV) was cloned and expressed as a recombinant protein in Escherichia coli BL21 (DE3) …

[HTML][HTML] Preparation and characterization of a single-domain antibody specific for the porcine epidemic diarrhea virus spike protein

F Bao, L Wang, X Zhao, T Lu, AM Na, X Wang, J Cao… - AMB Express, 2019 - Springer
Porcine epidemic diarrhea (PED) is a diarrheal disease of swine caused by porcine
epidemic diarrhea virus (PEDV). It is characterized by acute watery diarrhea, dehydration …

[HTML][HTML] The S2 glycoprotein subunit of porcine epidemic diarrhea virus contains immunodominant neutralizing epitopes

FA Okda, S Lawson, A Singrey, J Nelson, KS Hain… - Virology, 2017 - Elsevier
The porcine epidemic diarrhea virus (PEDV) spike (S) protein is the major target of
neutralizing antibodies against PEDV. Here immunodominant neutralizing epitopes of PEDV …

[HTML][HTML] Expression and immunogenicity of non-structural protein 8 of porcine epidemic diarrhea virus

H Chen, J Wan, M Wei, P Liu, L Kong… - Veterinary Research …, 2024 - ncbi.nlm.nih.gov
The non-structural protein (nsp) 8 of the porcine epidemic diarrhea virus (PEDV) is highly
stable across different PEDV strains and plays an important role in PEDV virulence. In …

[HTML][HTML] A New Neutralization Epitope in the Spike Protein of Porcine Epidemic Diarrhea Virus

J Liu, H Shi, J Chen, X Zhang, D Shi, Z Ji… - International Journal of …, 2022 - mdpi.com
Porcine epidemic diarrhea virus (PEDV) infects piglets and causes serious diarrhea as well
as vomiting, dehydration, and death. The trimeric S protein plays a crucial role in the …

Prokaryotic expression of truncated S1 protein of porcine epidemic diarrhea virus and production of monoclonal antibodies to recombinant protein

J Wang, J Chen, F Wei, Y Dong, L Zhu… - Monoclonal …, 2015 - liebertpub.com
Monoclonal antibodies are known to have several applications in clinical diagnosis and
therapy. In the present study, the truncated S1 gene, encoding the exterior of the viral spike …