Only kosmotrope anions trigger fibrillization of the recombinant core spidroin eADF4(C16) from Araneus diadematus

V Hovanová, A Hovan, M Humenik, E Sedlák - Protein Science, 2023 - Wiley Online Library
Recombinant core spidroin eADF4 (C16) has received increasing attention due to its ability
to form micro‐and nano‐structured scaffolds, which are based on nanofibrils with great …

Tyrosine Mutation in the Characteristic Motif of the Amorphous Region of Spidroin for Self-Assembly Capability Enhancement

Z Chen, C Cheng, L Liu, B Lin, Y Xiong, W Zhu… - ACS …, 2024 - ACS Publications
Spidroin, with robust mechanical performance and good biocompatibility, could fulfill broad
applications in material science and biomedical fields. Development of miniature spidroin …

Wet-spinning synthetic fibers from aggregate glue: aggregate spidroin 1 (AgSp1)

X Li, J Mi, R Wen, J Zhang, Y Cai… - ACS Applied Bio …, 2020 - ACS Publications
Spidroin has the potential of wide applications in the biomedicine field as a natural
biomaterial. Various synthetic fibers with outstanding mechanical properties have been …

Conformational stability and interplay of helical N-and C-terminal domains with implications on major ampullate spidroin assembly

J Bauer, T Scheibel - Biomacromolecules, 2017 - ACS Publications
Major ampullate spidroin (MaSp) assembly starts in the abdomen of the spider, where
spidroins are stored as a liquid dope at a high concentration. The dope is squeezed into the …

Engineered a novel pH-sensitive short major ampullate spidroin

C Zhang, J Mi, H Qi, J Huang, S Liu, L Zhang… - International journal of …, 2020 - Elsevier
The pH diversification has been proved as an important factor affecting the self-assembly of
spidroin. Herein, we constructed a novel spider silk protein (NT-MaSp1s-CT) with the pH …

A self-assembling peptide RADA16-I integrated with spider fibroin uncrystalline motifs

L Sun, X Zhao - International Journal of Nanomedicine, 2012 - Taylor & Francis
Mechanical strength of nanofiber scaffolds formed by the self-assembling peptide RADA16-I
or its derivatives is not very good and limits their application. To address this problem, we …

Effect of functionalization on the self-assembling propensity of β-sheet forming peptides

F Taraballi, M Campione, A Sassella, A Vescovi… - Soft matter, 2009 - pubs.rsc.org
The mechanism underlying self-assembly of short peptides has not been fully understood
despite the fact that a few decades have passed since their serendipitous discovery …

Novel highly soluble chimeric recombinant spidroins with high yield

Q Jia, R Wen, Q Meng - International Journal of Molecular Sciences, 2020 - mdpi.com
Spider silk has been a hotspot in the study of biomaterials for more than two decades due to
its outstanding mechanical properties. Given that spiders cannot be farmed, and their low …

High intracellular stability of the spidroin N‐terminal domain in spite of abundant amyloidogenic segments revealed by in‐cell hydrogen/deuterium exchange mass …

M Kaldmäe, A Leppert, G Chen, M Sarr… - The FEBS …, 2020 - Wiley Online Library
Proteins require an optimal balance of conformational flexibility and stability in their native
environment to ensure their biological functions. A striking example is spidroins, spider silk …

Properties of two spliceoforms of major ampullate spidroin 1 reveal unique functions of N-linker region

K Wang, R Wen, Q Meng - International journal of biological …, 2020 - Elsevier
Spiders produce diverse silk fibers with distinct properties for daily survival. Among these
silk fibers, dragline silk spun by major ampullate gland is used for bridgelines and web radii …