Mass activated droplet sorting (mads) enables high‐throughput screening of enzymatic reactions at nanoliter scale

DA Holland‐Moritz, MK Wismer… - Angewandte Chemie …, 2020 - Wiley Online Library
Microfluidic droplet sorting enables the high‐throughput screening and selection of water‐in‐
oil microreactors at speeds and volumes unparalleled by traditional well‐plate approaches …

Recent advances on sorting methods of high-throughput droplet-based microfluidics in enzyme directed evolution

X Fu, Y Zhang, Q Xu, X Sun, F Meng - Frontiers in Chemistry, 2021 - frontiersin.org
Droplet-based microfluidics has been widely applied in enzyme directed evolution (DE), in
either cell or cell-free system, due to its low cost and high throughput. As the isolation …

Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS)

F Gielen, R Hours, S Emond… - Proceedings of the …, 2016 - National Acad Sciences
Ultrahigh-throughput screening, in which members of enzyme libraries compartmentalized
in water-in-oil emulsion droplets are assayed, has emerged as a powerful format for directed …

Ultra-high-throughput absorbance-activated droplet sorting for enzyme screening at kilohertz frequencies

EJ Medcalf, M Gantz, TS Kaminski… - Analytical …, 2023 - ACS Publications
Droplet microfluidics is a valuable method to “beat the odds” in high throughput screening
campaigns such as directed evolution, where valuable hits are infrequent and large library …

Design and construction of a microfluidics workstation for high-throughput multi-wavelength fluorescence and transmittance activated droplet analysis and sorting

J Panwar, A Autour, CA Merten - Nature Protocols, 2023 - nature.com
Droplet microfluidics has revolutionized quantitative high-throughput bioassays and
screening, especially in the field of single-cell analysis where applications include cell …

Microdroplet-assisted screening of biomolecule production for metabolic engineering applications

EK Bowman, HS Alper - Trends in biotechnology, 2020 - cell.com
Success in synthetic biology and metabolic engineering is quickly becoming 'test'limited
within the design–build–test cycle. Commonly used methods for high-throughput screening …

Coupling microdroplet microreactors with mass spectrometry: reading the contents of single droplets online

LM Fidalgo, G Whyte, BT Ruotolo… - Angewandte …, 2009 - Wiley Online Library
Emulsions created inside microfluidic devices have generated significant interest in recent
years because of their ability to compartmentalize reactions and assays on a microfluidic …

UV–Vis spectra-activated droplet sorting for label-free chemical identification and collection of droplets

TA Duncombe, A Ponti, FP Seebeck… - Analytical …, 2021 - ACS Publications
We introduce the UV–vis spectra-activated droplet sorter (UVADS) for high-throughput label-
free chemical identification and enzyme screening. In contrast to previous absorbance …

Sensitive, high throughput detection of proteins in individual, surfactant-stabilized picoliter droplets using nanoelectrospray ionization mass spectrometry

CA Smith, X Li, TH Mize, TD Sharpe… - Analytical …, 2013 - ACS Publications
Droplet-based fluidics is emerging as a powerful platform for single cell analysis, directed
evolution of enzymes, and high throughput screening studies. Due to the small amounts of …

[HTML][HTML] Dilution-free analysis from picoliter droplets by Nano-ESI MS

RT Kelly, JS Page, I Marginean, K Tang… - … (International ed. in …, 2009 - ncbi.nlm.nih.gov
The amount of sample required for a chemical analysis is frequently determined not by
instrument sensitivity but by the ability to isolate, prepare and deliver trace analytes to the …