Mass activated droplet sorting (mads) enables high‐throughput screening of enzymatic reactions at nanoliter scale
DA Holland‐Moritz, MK Wismer… - Angewandte Chemie …, 2020 - Wiley Online Library
Microfluidic droplet sorting enables the high‐throughput screening and selection of water‐in‐
oil microreactors at speeds and volumes unparalleled by traditional well‐plate approaches …
oil microreactors at speeds and volumes unparalleled by traditional well‐plate approaches …
Recent advances on sorting methods of high-throughput droplet-based microfluidics in enzyme directed evolution
Droplet-based microfluidics has been widely applied in enzyme directed evolution (DE), in
either cell or cell-free system, due to its low cost and high throughput. As the isolation …
either cell or cell-free system, due to its low cost and high throughput. As the isolation …
Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS)
Ultrahigh-throughput screening, in which members of enzyme libraries compartmentalized
in water-in-oil emulsion droplets are assayed, has emerged as a powerful format for directed …
in water-in-oil emulsion droplets are assayed, has emerged as a powerful format for directed …
Ultra-high-throughput absorbance-activated droplet sorting for enzyme screening at kilohertz frequencies
EJ Medcalf, M Gantz, TS Kaminski… - Analytical …, 2023 - ACS Publications
Droplet microfluidics is a valuable method to “beat the odds” in high throughput screening
campaigns such as directed evolution, where valuable hits are infrequent and large library …
campaigns such as directed evolution, where valuable hits are infrequent and large library …
Design and construction of a microfluidics workstation for high-throughput multi-wavelength fluorescence and transmittance activated droplet analysis and sorting
J Panwar, A Autour, CA Merten - Nature Protocols, 2023 - nature.com
Droplet microfluidics has revolutionized quantitative high-throughput bioassays and
screening, especially in the field of single-cell analysis where applications include cell …
screening, especially in the field of single-cell analysis where applications include cell …
Microdroplet-assisted screening of biomolecule production for metabolic engineering applications
Success in synthetic biology and metabolic engineering is quickly becoming 'test'limited
within the design–build–test cycle. Commonly used methods for high-throughput screening …
within the design–build–test cycle. Commonly used methods for high-throughput screening …
Coupling microdroplet microreactors with mass spectrometry: reading the contents of single droplets online
Emulsions created inside microfluidic devices have generated significant interest in recent
years because of their ability to compartmentalize reactions and assays on a microfluidic …
years because of their ability to compartmentalize reactions and assays on a microfluidic …
UV–Vis spectra-activated droplet sorting for label-free chemical identification and collection of droplets
TA Duncombe, A Ponti, FP Seebeck… - Analytical …, 2021 - ACS Publications
We introduce the UV–vis spectra-activated droplet sorter (UVADS) for high-throughput label-
free chemical identification and enzyme screening. In contrast to previous absorbance …
free chemical identification and enzyme screening. In contrast to previous absorbance …
Sensitive, high throughput detection of proteins in individual, surfactant-stabilized picoliter droplets using nanoelectrospray ionization mass spectrometry
Droplet-based fluidics is emerging as a powerful platform for single cell analysis, directed
evolution of enzymes, and high throughput screening studies. Due to the small amounts of …
evolution of enzymes, and high throughput screening studies. Due to the small amounts of …
[HTML][HTML] Dilution-free analysis from picoliter droplets by Nano-ESI MS
The amount of sample required for a chemical analysis is frequently determined not by
instrument sensitivity but by the ability to isolate, prepare and deliver trace analytes to the …
instrument sensitivity but by the ability to isolate, prepare and deliver trace analytes to the …